| Clinical significance of TC21 overexpression in oral cancer. | |
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MedLine Citation:
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PMID: 20040018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In search of novel molecular markers for oral cancer, we reported increased levels of TC21/R-Ras2 transcripts in oral squamous cell carcinoma by differential display. The aim of this study was to determine the clinical significance of TC21 in oral cancer. METHODS: Immunohistochemical analysis of TC21 protein expression was carried out in 120 leukoplakias, 83 OSCCs and 30 non-malignant tissues, confirmed by immunoblotting, and correlated with clinicopathological parameters as well as disease prognosis. Co-immunoprecipitation assays were carried out to identify the interaction partners of TC21 protein in oral cancer cells and tissues. RESULTS: TC21 nuclear expression increased from normal oral tissues to leukoplakia and frank malignancy (P < 0.001). TC21 overexpression was observed in 74.2% leukoplakia with no dysplasia, 75.9% dysplasias and 79.5% OSCCs in comparison with normal oral tissues. Receiver operating characteristic analysis showed that the area-under-the curve values were 0.895, 0.885, and 0.919, while the positive predictive values were 95.8%, 95.6%, and 97.1%, for nuclear immunostaining for normal versus leukoplakia with no dysplasia, leukoplakic lesions with dysplasia, and OSCCs, respectively. Immunoblotting confirmed overexpression of TC21 in oral lesions. Using co-immunoprecipitation assays, we showed interactions of TC21 with Erk2, PI3-K, 14-3-3zeta and 14-3-3sigma proteins in oral cancer cells. CONCLUSION: Our findings suggested that alteration in TC21 expression is an early event in oral cancer and correlates with poor prognosis of OSCCs. TC21 interactions with Erk2, PI3-K, 14-3-3zeta and 14-3-3sigma proteins in oral cancer cells and tissues suggests the involvement of TC21 in signaling pathways in oral cancer. |
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Authors:
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Muzafar A Macha; Ajay Matta; Uma Sriram; Alok Thakkar; N K Shukla; Siddhartha Datta Gupta; Ranju Ralhan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-16 |
Journal Detail:
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Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology Volume: 39 ISSN: 1600-0714 ISO Abbreviation: J. Oral Pathol. Med. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-20 Completed Date: 2010-11-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8911934 Medline TA: J Oral Pathol Med Country: Denmark |
Other Details:
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Languages: eng Pagination: 477-85 Citation Subset: D; IM |
Affiliation:
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Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India. |
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism 14-3-3 Proteins / metabolism Adult Aged Carcinoma, Squamous Cell / genetics, metabolism*, pathology Cell Line, Tumor Cell Nucleus / metabolism Disease-Free Survival Exonucleases / metabolism Gene Expression Humans Immunoprecipitation Kaplan-Meiers Estimate Leukoplakia, Oral / genetics, metabolism*, pathology Membrane Proteins / biosynthesis*, genetics Middle Aged Mitogen-Activated Protein Kinase 1 / metabolism Monomeric GTP-Binding Proteins / biosynthesis*, genetics Mouth Neoplasms / genetics, metabolism*, pathology Prognosis ROC Curve Signal Transduction Tumor Markers, Biological / biosynthesis*, genetics, metabolism Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/14-3-3 Proteins; 0/Membrane Proteins; 0/Tumor Markers, Biological; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.24/MAPK1 protein, human; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 3.1.-/Exonucleases; EC 3.1.-/SFN protein, human; EC 3.6.1/RRAS2 protein, human; EC 3.6.5.2/Monomeric GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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