| Clinical retroviral vector production: step filtration using clinically approved filters improves titers. | |
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MedLine Citation:
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PMID: 11175310 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Production of retroviral vectors for clinical use requires removal of cells and cellular debris. We combined a series of filters of decreasing pore size using commercially available blood banking filters approved for clinical use. The collection bag and filters can be connected to create a sterile, closed system using clinically approved tubing and sealing systems. Even when challenged with a large number of vector producer cells (2.38 x 10(9)cells), no viable cells are passed through the system. The step filtration system developed minimizes the titer reduction associated with filtration, provides rapid flow rates, and was cost effective when filtering volumes in excess of 2 liters. |
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Authors:
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L Reeves; K Cornetta |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gene therapy Volume: 7 ISSN: 0969-7128 ISO Abbreviation: Gene Ther. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-04-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421525 Medline TA: Gene Ther Country: England |
Other Details:
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Languages: eng Pagination: 1993-8 Citation Subset: IM |
Affiliation:
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Division of Hematology/Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Filtration
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instrumentation*,
methods Gene Therapy / instrumentation* Genetic Vectors* Humans Retroviridae / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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P50 DK49218/DK/NIDDK NIH HHS; PHS P01 HL53586/PH/PHPPO CDC HHS; U42 RR11148/RR/NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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