Document Detail


Clinical microscopy of the cornea utilizing optical sectioning and a high-numerical-aperture objective.
MedLine Citation:
PMID:  8350157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A doublet contact element was added to a long-working-distance objective to increase the numerical aperture to 0.75 and to maintain the focus during in vivo examination of the eye. Optical sectioning by use of confocal slits permits visualization of weakly scattering structures within the cornea. With photographic film and a 1/60-s exposure time to limit the effect of eye movement, an effective optical section half-thickness of approximately 20 microns was realized. Structures observed in the cornea include epithelial cells (surface, wing, and basal cells), nerve-fiber bundles in the subepithelial region, keratocytes and inflammatory cells in the stroma, and endothelial cells.
Authors:
C J Koester; J D Auran; H D Rosskothen; G J Florakis; R B Tackaberry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the Optical Society of America. A, Optics and image science     Volume:  10     ISSN:  0740-3232     ISO Abbreviation:  J Opt Soc Am A     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-13     Completed Date:  1993-09-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8402086     Medline TA:  J Opt Soc Am A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1670-9     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Columbia University, New York, New York 10032.
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MeSH Terms
Descriptor/Qualifier:
Cornea / cytology*,  innervation
Corneal Stroma / cytology
Endothelium, Corneal / cytology
Epithelial Cells
Humans
Male
Microscopy / instrumentation,  methods*
Middle Aged
Nerve Fibers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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