Document Detail


Clinical manifestations and anti-phospholipid antibodies in 712 patients with systemic lupus erythematosus: evaluation of two diagnostic assays.
MedLine Citation:
PMID:  23159889     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objectives. To evaluate the agreement and performance of two tests for aPLs with regard to association with manifestations of the APS in patients with SLE.Methods. We investigated 712 SLE patients and 280 population controls. Cardiolipin and β(2) glycoprotein-I antibodies were measured with routine ELISA and a new automated method. Three positivity cut-offs (99%, 90% of controls and recommended cut-off by manufacturers) were used. Associations with previous thrombotic events, thrombocytopenia and, in a subgroup of patients, obstetric morbidity (n = 296) were evaluated. Results were compared with the LA test, performed in 380 patients.Results. Inter-test agreement was moderate (demonstrated by κ-values 0.16-0.71). Performance of the two tests was similar: at the 99th percentile cut-off, sensitivity for any thrombotic event ranged from 3.7% to 24.8%, while specificity was 84.7-97.7%. Regardless of assay, IgG isotypes were associated with venous thrombosis and ischaemic cerebrovascular disease, whereas aPLs of IgM isotype were weakly associated with ischaemic heart disease. Associations were greatly affected by aPL level. LA performed better than the specific aPL tests. LA was associated with any thrombotic event, odds ratio 5.4 (95% CI 3.1, 9.4), while the specific aPL tests ranged from non-significant to an odds ratio of 1.9 (95% CI 1.03, 3.4) using criteria cut-off. LA was also convincingly associated with other APS manifestations.Conclusion. In relation to thrombotic manifestations, there was moderate agreement but no clear advantages when comparing a routine aPL ELISA with an automated method. APL isotype and titre as well as LA positivity are important for risk assessment in SLE patients.
Authors:
Anna Vikerfors; Anna-Britta Johansson; Johanna T Gustafsson; Andreas Jönsen; Dag Leonard; Agneta Zickert; Gunnel Nordmark; Gunnar Sturfelt; Anders Bengtsson; Lars Rönnblom; Iva Gunnarsson; Kerstin Elvin; Elisabet Svenungsson
Related Documents :
24621429 - Auto-segmentation of low-risk clinical target volume for head and neck radiation therapy.
644799 - Evaluation of the optical refractometer for lamb serum immunoglobulin estimation.
18841439 - Major inter-laboratory variations in psa testing practices: results from national surve...
5296399 - Testing blackfly larvicides in the laboratory and in streams.
23761879 - Obesity and worsening of chronic venous disease and joint mobility.
3618549 - Diagnosis of infective and inflammatory disorders by flow cytometric analysis of blood ...
25157919 - Evaluation of the nuclisens easyq v2.0 assay in comparison with the roche amplicor v1.5...
9429859 - Biochemical testing of thyroid function.
25148559 - Performing gait analysis within the timed up & go assessment test: comparison of atug t...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-18
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  -     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Unit of Rheumatology, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, Department of Clinical Immunology and Transfusion Medicine, Unit of Clinical Immunology, Karolinska Institutet, Department of Clinical Sciences, Section of Rheumatology, Lund University, Skane University Hospital and Department of Medical Sciences, Section of Rheumatology, Uppsala University, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Analysis of Mammalian Alcohol Dehydrogenase 5 (ADH5) Characterization of rat ADH5 with comparisons t...
Next Document:  Normalization of NF-?B Activity in Dorsal Root Ganglia Neurons Cultured from Diabetic Rats Reverses ...