Document Detail

Clinical and laboratory characteristics of patients having amyloidogenic transthyretin deposition in osteoarthritic knee joints.
MedLine Citation:
PMID:  24390749     Owner:  NLM     Status:  In-Data-Review    
Objective: Our aim was to investigate clinical and laboratory characteristics of osteoarthritic patients who had amyloid deposition in their knee joints. Methods: Synovial membranes were obtained from 36 patients with knee osteoarthritis (OA) who underwent joint replacement surgery. From this sample, the diagnosis of amyloid was determined by Congo red staining, which demonstrated apple-green birefringence under a polarized microscope. All synovial membranes were immunohistochemically characterized for the expressions of amyloid immunoglobulin light chain (AL-κ and AL-λ), serum amyloid-A (SAA), amyloidogenic transthyretin (ATTR), and amyloidogenic β2-microglobulin (Aβ2M). Matrix-assisted laser desorption-ionizaton/time of flight mass spectrometry (MALDI-TOF MS) was used to analyze transthyretin (TTR) isoforms in the serum of each patient. Results: Nine cases (25%) were found to be amyloid-positive. Immunohistochemically, eight cases (88.9%) had ATTR deposition, and one sample (11.1%) was shown to be AL-κ-positive. MALDI-TOF MS identified that the TTR in the serum of the patients was unmodified wild-type TTR, TTR-Cys-S-S-Cys, and TTR-Cys-S-S-CysGly. The age at surgery and the disease duration were significantly higher in the ATTR-positive group than in the ATTR-negative group. Knee score and function score were significantly lower in the ATTR-positive group than in the ATTR-negative group. Conclusions: Amyloid deposition in synovial membranes of OA patients was found to be ATTR and AL-κ. TTR in the serum of the patients was unmodified wild-type TTR together with two isoforms. The high age at surgery, long disease duration, and a deteriorated knee function were associated with ATTR amyloid deposition in the osteoarthritic knee joints.
Ya-Jun Gu; Peng Ge; Yun Mu; Jin-Hai Lu; Fang Zheng; Xu-Guo Sun
Related Documents :
2079469 - Aetiology of diabetic foot ulceration.
17419799 - Interventions to enhance patient compliance with leg ulcer treatment: a review of the l...
12020629 - Reflux esophagitis and hiatal hernia as concomitant abnormality in patients presenting ...
17035449 - Nontraumatic disruption of the fibrocartilaginous trachea: causes and clinical outcomes.
3994179 - Peptic ulcer disease and the nonsteroidal anti-inflammatory drugs.
22327689 - Near infrared spectroscopy as possible non-invasive monitor of slow vasogenic icp waves.
9873779 - Rehabilitation in haemophilia--options in the developing world.
17336759 - Prospective evaluation of aortic dimensions in turner syndrome: a 2-dimensional echocar...
282809 - Leukemia in radiation-treated patients: cytogenetic studies in eight cases.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  15     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-01-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  92-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparative pharmacokinetics of borneol in cerebral ischemia-reperfusion and sham-operated rats.
Next Document:  Molecular identification of Sporothrix clinical isolates in China.