Document Detail

Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis.
MedLine Citation:
PMID:  23397597     Owner:  NLM     Status:  Publisher    
Actinic keratoses (AKs) is a keratinocytic neoplasm that typically develops on the face of elderly patients. Little is known regarding the clinical, dermatoscopic and immunohistochemical assessments of AK using topical diclofenac therapy. We sought to determine these assessments and evaluate the efficacy of topical diclofenac gel in AK. In this prospective, open-label study, 44 patients with 66 AKs were treated for 12 weeks with topically applied diclofenac (3 % gel in 2.5 % hyaluronic acid). Immunohistopathologic analyses were performed before and after diclofenac treatment using epidermal stem cell markers such as Cytokeratin 15 (CK15), Cytokeratin 19 (CK19) and p63, in addition to proliferation markers (Bcl-2, Ki-67). Diclofenac gel was found to be effective in AK, including the hyperkeratotic type. Surprisingly, complete remission was observed at a significantly higher rate in Grade 3 lesions (p = 0.017). However, imunohistochemical and histopathologic examinations revealed that 12-week treatment periods may not be sufficient to fully cure AK. The immunohistochemical analyses revealed no change in the expression levels of CK15, CK19 and Bcl-2 following diclofenac therapy. However, the expression of Ki-67 (p = 0.042) and p63 (p = 0.030) exhibited a significant decrease after therapy. Dermatoscopy is an effective method for diagnosis of AK, and topical diclofenac sodium gel was found as an effective additional treatment modality. Since positive histopathological findings were detected in some patients even with significant remission, a 12-week treatment period should be extended even in patients presenting with positive clinical response. Importantly, anti-proliferative effects of diclofenac were demonstrated by decreased Ki-67 and p63 expression levels.
Mutlu Cayirli; Osman Köse; Murat Demiriz
Related Documents :
24179567 - Symptom improvement after helicobacter pylori eradication in patients with functional d...
25012927 - Comparisons of three anterior cervical surgeries in treating cervical spondylotic myelo...
17035947 - Bravo i: a pilot study of vascular brachytherapy in polytetrafluoroethylene dialysis ac...
10027357 - Reintervention and mortality after infrainguinal reconstructive surgery for leg ischaemia.
6180407 - Clinical and electrophysiological properties of a new temporary pacemaker lead after op...
11689137 - Possible clinically significant interaction of itraconazole plus rifampin.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-9
Journal Detail:
Title:  Archives of dermatological research     Volume:  -     ISSN:  1432-069X     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Dermatology Services, Ağrı Military Hospital, Ağrı, Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Endothelial NOS (NOS3) impairs myocardial function in developing sepsis.
Next Document:  A novel seven-base deletion of the CTSC gene identified in a Hungarian family with Papillon-Lefèvre...