Document Detail


Clinical and clinicopathologic effects of plateletpheresis on healthy donor dogs.
MedLine Citation:
PMID:  18564392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The safety and feasibility of plateletpheresis using a commercially available apheresis system (COBE Spectra, Gambro BCT) were evaluated in donor dogs, with characterization of its clinical and clinicopathologic effects. STUDY DESIGN AND METHODS: Fourteen adult dogs (18-27.7 kg) underwent a plateletpheresis procedure. Complete blood counts were obtained at baseline, 2 hours after apheresis, and daily for 1 week. Blood was collected every 15 minutes for acid-base and electrolyte analysis and measurement of serum citrate concentration. Dogs were monitored by continuous electrocardiogram and indirect blood pressure measurement. All dogs received prophylactic calcium (Ca) supplementation (10% Ca gluconate infusion at 15 mL/hr [139.5 mg Ca ion/hr]; the rate was increased based on serial measurement of ionized Ca [iCa] concentration). RESULTS: A high-quality platelet concentrate (PC) was collected, with a mean total yield of 3.3 x 10(11) platelets (PLTs). The mean donor PLT count decreased from 356 x 10(9) to 159 x 10(9) per L after apheresis. The procedure was generally well tolerated, with no evidence of hypotension. Serum citrate concentration progressively increased, causing the ionized magnesium concentration to decrease by 45 percent and iCa to decrease to less than 1 mmol per L (mean baseline, 1.2 mmol/L) in 10 dogs, despite receiving 0.9 mg of Ca ion per mL acid-citrate-dextrose formula A. Lip licking was noted in 3 dogs, and generalized tremors and ventricular ectopy were noted in 1 dog. CONCLUSION: Canine plateletpheresis using the COBE Spectra is a feasible option for production of a PC. Hypocalcemia, however, is a potential serious adverse effect of plateletpheresis in dogs. Ca supplementation is recommended to limit clinical signs of hypocalcemia during the procedure.
Authors:
Mary Beth Callan; Elizabeth H Appleman; Frances S Shofer; Nicola J Mason; Benjamin M Brainard; Reid P Groman
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-06-28
Journal Detail:
Title:  Transfusion     Volume:  48     ISSN:  1537-2995     ISO Abbreviation:  Transfusion     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2008-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2214-21     Citation Subset:  IM    
Affiliation:
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6010, USA. callan@vet.upenn.edu
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MeSH Terms
Descriptor/Qualifier:
Acid-Base Equilibrium
Animals
Blood Donors*
Blood Pressure
Calcium / administration & dosage,  blood
Citric Acid / blood
Dogs
Electrocardiography
Feasibility Studies
Female
Gluconates / administration & dosage
Hypocalcemia / prevention & control*
Male
Plateletpheresis / adverse effects,  methods*,  veterinary*
Grant Support
ID/Acronym/Agency:
RR02512/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Gluconates; 526-95-4/gluconic acid; 7440-70-2/Calcium; 77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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