| Clinical and clinicopathologic effects of plateletpheresis on healthy donor dogs. | |
| | |
MedLine Citation:
|
PMID: 18564392 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: The safety and feasibility of plateletpheresis using a commercially available apheresis system (COBE Spectra, Gambro BCT) were evaluated in donor dogs, with characterization of its clinical and clinicopathologic effects. STUDY DESIGN AND METHODS: Fourteen adult dogs (18-27.7 kg) underwent a plateletpheresis procedure. Complete blood counts were obtained at baseline, 2 hours after apheresis, and daily for 1 week. Blood was collected every 15 minutes for acid-base and electrolyte analysis and measurement of serum citrate concentration. Dogs were monitored by continuous electrocardiogram and indirect blood pressure measurement. All dogs received prophylactic calcium (Ca) supplementation (10% Ca gluconate infusion at 15 mL/hr [139.5 mg Ca ion/hr]; the rate was increased based on serial measurement of ionized Ca [iCa] concentration). RESULTS: A high-quality platelet concentrate (PC) was collected, with a mean total yield of 3.3 x 10(11) platelets (PLTs). The mean donor PLT count decreased from 356 x 10(9) to 159 x 10(9) per L after apheresis. The procedure was generally well tolerated, with no evidence of hypotension. Serum citrate concentration progressively increased, causing the ionized magnesium concentration to decrease by 45 percent and iCa to decrease to less than 1 mmol per L (mean baseline, 1.2 mmol/L) in 10 dogs, despite receiving 0.9 mg of Ca ion per mL acid-citrate-dextrose formula A. Lip licking was noted in 3 dogs, and generalized tremors and ventricular ectopy were noted in 1 dog. CONCLUSION: Canine plateletpheresis using the COBE Spectra is a feasible option for production of a PC. Hypocalcemia, however, is a potential serious adverse effect of plateletpheresis in dogs. Ca supplementation is recommended to limit clinical signs of hypocalcemia during the procedure. |
| | |
Authors:
|
Mary Beth Callan; Elizabeth H Appleman; Frances S Shofer; Nicola J Mason; Benjamin M Brainard; Reid P Groman |
Publication Detail:
|
Type: Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-06-28 |
Journal Detail:
|
Title: Transfusion Volume: 48 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2008 Oct |
Date Detail:
|
Created Date: 2008-10-20 Completed Date: 2008-11-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
|
Languages: eng Pagination: 2214-21 Citation Subset: IM |
Affiliation:
|
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6010, USA. callan@vet.upenn.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acid-Base Equilibrium Animals Blood Donors* Blood Pressure Calcium / administration & dosage, blood Citric Acid / blood Dogs Electrocardiography Feasibility Studies Female Gluconates / administration & dosage Hypocalcemia / prevention & control* Male Plateletpheresis / adverse effects, methods*, veterinary* |
| Grant Support | |
ID/Acronym/Agency:
|
RR02512/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Gluconates; 526-95-4/gluconic acid; 7440-70-2/Calcium; 77-92-9/Citric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Leukodepletion of autologous whole blood has no impact on perioperative infection rate and length of...
Next Document: Fatal group C streptococcal infection due to transfusion of a bacterially contaminated pooled platel...