Document Detail

Clinical usefulness of cell-based indirect immunofluorescence assay for the detection of aquaporin-4 antibodies in neuromyelitis optica spectrum disorder.
MedLine Citation:
PMID:  22950068     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The presence of antibodies to aquaporin-4 (AQP4) has been identified as a key characteristic of neuromyelitis optica spectrum disorder (NMOSD), an autoimmune inflammatory demyelinating central nervous system (CNS) disorder. We evaluated the performance of a cell-based indirect immunofluorescence assay (CIIFA) for detecting AQP4 antibodies using antigen prepared with a recombinant AQP4 peptide transfection technique and assessed the usefulness of CIIFA for diagnosis of NMOSD in routine clinical practice.
METHODS: Forty-six serum samples from 36 patients as a comparison set and another 101 patients enrolled consecutively from a neurology clinic were included. CIIFA and fluorescence immunoprecipitation assays (FIPA) were performed. CIIFA was performed at 2 different institutions for comparison purposes.
RESULTS: CIIFA and FIPA sensitivity in the comparison set was 86% and 79% in neuromyelitis optica (NMO) patients and 55% and 36% in high-risk NMO patients, respectively. The semiquantitative titer measured by CIIFA correlated well with the arbitrary unit (fluorescence units [FU]) derived from FIPA (r=0.66). Titers measured by CIIFA and FIPA were elevated in NMO patients compared to high-risk NMO patients (1:240 vs. 1:180 and 8,390 vs. 4,059 FU, respectively). The frequency of AQP4 antibody detection by CIIFA in 101 consecutively enrolled patients was 100% in NMO and 23% in high-risk NMO patients, while only 4.6% in control patients, including those with multiple sclerosis.
CONCLUSIONS: Detection of AQP4 antibodies by CIIFA provides sensitive and highly specific diagnostic information for NMO and high-risk NMO patients, which can be used to differentiate these conditions from other demyelinating CNS diseases.
Eun-suk Kang; Ju-Hong Min; Kwang Ho Lee; Byoung Joon Kim
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-13
Journal Detail:
Title:  Annals of laboratory medicine     Volume:  32     ISSN:  2234-3814     ISO Abbreviation:  Ann Lab Med     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-05     Completed Date:  2013-01-11     Revised Date:  2014-05-30    
Medline Journal Info:
Nlm Unique ID:  101571172     Medline TA:  Ann Lab Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  331-8     Citation Subset:  IM    
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MeSH Terms
Antibodies / blood*
Aquaporin 4 / immunology*
Fluorescent Antibody Technique, Indirect*
Middle Aged
Neuromyelitis Optica / diagnosis*
Reagent Kits, Diagnostic
Reg. No./Substance:
0/Antibodies; 0/Aquaporin 4; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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