Document Detail

Clinical evidence of the association between serum perioperative changes in xanthine metabolizing enzymes activity and early post-transplant kidney allograft function.
MedLine Citation:
PMID:  20829075     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Xanthine oxidoreductase (XOR) and its active forms, dehydrogenase (XD) and oxidase (XO), act as double-edged swords during ischemia-reperfusion injury. On the one hand, their action generates antioxidants, such as uric acid (UA); however, they may strongly enhance production of free radicals. In this study, we examined the association between post-transplant graft function and perioperative xanthine metabolizing enzymes (XME) activity in kidney transplant recipients divided into early (EGF), slow (SGF), and delayed graft function (DGF) groups.
STUDY DESIGN: XME activity and UA levels were measured in blood samples collected directly before and during the first and fifth minutes of reperfusion.
RESULTS: Results demonstrated an increase in XO and XOR activity in all groups; however, these parameters were lower in the EGF than in the DGF group (p < 0.005; p < 0.05). XD activity increased in SGF and DGF patients (p = 0.01); nevertheless, the XD/total XOR coefficient decreased only in DGF individuals (p = 0.0007). XME sensitivity, specificity, and positive and negative predictive values in discriminating SGF/DGF from EGF were 73.3% to 78%, 54% to 62.5%, 76% to 78.6%, and 56.5%, respectively. Moreover, mixed model analysis revealed that recipients classified according to results of XOR(5) and XO(5) significantly differ in 1-year post-transplant allograft function (p = 0.04 and p = 0.02, respectively), but not in the frequency of acute rejection episodes (p = 0.66 and p = 0.90, respectively).
CONCLUSIONS: During renal transplantation, significant changes in XME occur that are associated with early post-transplant graft function and have potential value to discern between EGF and SGF/DGF.
Barbara Dołęgowska; Wojciech Błogowski; Leszek Domański
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Journal of the American College of Surgeons     Volume:  211     ISSN:  1879-1190     ISO Abbreviation:  J. Am. Coll. Surg.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431305     Medline TA:  J Am Coll Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  587-95     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2010 American College of Surgeons. Published by Elsevier Inc. All rights reserved.
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Szczecin, Poland.
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MeSH Terms
Area Under Curve
Biological Markers / blood
Delayed Graft Function / enzymology*
Graft Survival / physiology
Kidney Transplantation / physiology*
Middle Aged
Multivariate Analysis
Oxidative Stress
Perioperative Period
Postoperative Period
Reperfusion Injury / enzymology
Retrospective Studies
Transplantation, Homologous / physiology
Uric Acid / blood*
Xanthine Dehydrogenase / blood*
Xanthine Oxidase / blood*
Reg. No./Substance:
0/Biological Markers; 69-93-2/Uric Acid; EC Dehydrogenase; EC Oxidase

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