| Click Peptide Concept: O-Acyl Isopeptide of Islet Amyloid Polypeptide as a Nonaggregative Precursor Molecule. | |
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MedLine Citation:
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PMID: 21538760 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The O-acyl isopeptide (1) of islet amyloid polypeptide (IAPP), which contains an ester moiety at both Ala8-Thr9 and Ser19-Ser20, was prepared by sequential segment condensation based on the O-acyl isopeptide method. Isopeptide 1 possessed nonaggregative properties, retaining its random coil structure under the acidic conditions; this suggests that the insertion of the O-acyl isopeptide structures in IAPP suppressed aggregation of the molecule. As a result of the rapid O-to-N acyl shift of 1 under neutral pH, in situ-formed IAPP adopted a random-coil structure at the start of the experiment, and then underwent conformational change to α-helix/β-sheet mixed structures as well as aggregation. The click peptide strategy with the nonaggregative precursor molecule 1 could be a useful experimental tool to identify the functions of IAPP, by overcoming the handling difficulties that arise from IAPP's intense and uncontrollable self-assembling nature. |
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Authors:
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Taku Yoshiya; Ayano Higa; Naoko Abe; Fukue Fukao; Tomomi Kuruma; Yuki Toda; Youhei Sohma; Yoshiaki Kiso |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-28 |
Journal Detail:
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Title: Chembiochem : a European journal of chemical biology Volume: - ISSN: 1439-7633 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-5-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100937360 Medline TA: Chembiochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Kyoto Pharmaceutical University, Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, Yamashina-ku, Kyoto 607-8412 (Japan), Fax: (+81) 75-595-4787. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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