| Cleaved forms of C-reactive protein are associated with platelet inhibition. | |
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MedLine Citation:
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PMID: 2419439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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C-reactive protein (CRP) is the prototypic acute phase reactant and serves clinically as a marker of inflammation and tissue destruction. When native CRP pentamer was incubated with Streptomyces griseus protease, a newly formed and transient ability to inhibit platelet aggregation stimulated by adenosine diphosphate or collagen was often elicited early during the course of enzymatic digestion. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses of the digests revealed that platelet inhibitory activity correlated with altered electrophoretic mobility and reductions in subunit and pentameric m.w. Minimally degraded forms of CRP were also isolated "de novo" from inflammatory fluids and, like their enzyme degraded counterparts, inhibited platelet activation. Dissociation of degraded CRP with SDS followed by the removal of SDS resulted in the separation fragments which inhibited platelet function. We propose that in a degradative environment, such as at sites of inflammation/tissue damage or through the action of serum proteases, CRP may transitorily down-regulate the platelet. |
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Authors:
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B A Fiedel; H Gewurz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 136 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-04-15 Completed Date: 1986-04-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2551-5 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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C-Reactive Protein
/
analysis,
metabolism*,
pharmacology Electrophoresis, Polyacrylamide Gel Endopeptidases Epitopes / analysis Humans Molecular Weight Peptide Fragments / isolation & purification*, pharmacology Platelet Aggregation / drug effects* Serine Endopeptidases |
| Grant Support | |
ID/Acronym/Agency:
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HL-00614/HL/NHLBI NIH HHS; HL-23457/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Peptide Fragments; 9007-41-4/C-Reactive Protein; EC 3.4.-/Endopeptidases; EC 3.4.21.-/Serine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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