Document Detail


Cleaved forms of C-reactive protein are associated with platelet inhibition.
MedLine Citation:
PMID:  2419439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
C-reactive protein (CRP) is the prototypic acute phase reactant and serves clinically as a marker of inflammation and tissue destruction. When native CRP pentamer was incubated with Streptomyces griseus protease, a newly formed and transient ability to inhibit platelet aggregation stimulated by adenosine diphosphate or collagen was often elicited early during the course of enzymatic digestion. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses of the digests revealed that platelet inhibitory activity correlated with altered electrophoretic mobility and reductions in subunit and pentameric m.w. Minimally degraded forms of CRP were also isolated "de novo" from inflammatory fluids and, like their enzyme degraded counterparts, inhibited platelet activation. Dissociation of degraded CRP with SDS followed by the removal of SDS resulted in the separation fragments which inhibited platelet function. We propose that in a degradative environment, such as at sites of inflammation/tissue damage or through the action of serum proteases, CRP may transitorily down-regulate the platelet.
Authors:
B A Fiedel; H Gewurz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  136     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-04-15     Completed Date:  1986-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2551-5     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
C-Reactive Protein / analysis,  metabolism*,  pharmacology
Electrophoresis, Polyacrylamide Gel
Endopeptidases
Epitopes / analysis
Humans
Molecular Weight
Peptide Fragments / isolation & purification*,  pharmacology
Platelet Aggregation / drug effects*
Serine Endopeptidases
Grant Support
ID/Acronym/Agency:
HL-00614/HL/NHLBI NIH HHS; HL-23457/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Epitopes; 0/Peptide Fragments; 9007-41-4/C-Reactive Protein; EC 3.4.-/Endopeptidases; EC 3.4.21.-/Serine Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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