| Cleavage of viral precursor proteins in vivo and in vitro. | |
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MedLine Citation:
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PMID: 4343549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of protease inhibitors causes the accumulation of very large polypeptides (polyprotein) in tissue culture cells infected with either poliovirus or echovirus 12. The effectiveness of the inhibitor varies, depending on the cell line chosen. In infected monkey kidney cells, polyprotein is not cleaved when a chymotrypsin inhibitor is added, but in infected HeLa cells a trypsin inhibitor is most effective. Therefore, at least a part of the proteolytic activity is supplied by the host cell. Extracted viral polyprotein can be cleaved in vitro by trypsin or chymotrypsin. As estimated by migration in sodium dodecyl sulfate gels and antigenicity, chymotrypsin cleavage of the poliovirus polyprotein yields fragments which are similar to the in vivo product. The polyprotein is not in soluble form but is attached to a fast-sedimenting, membrane-bound structure. Proteolytic activities in cell extracts were assayed using polyprotein as substrate, and infected and uninfected extracts produced qualitatively dissimilar cleavages. |
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Authors:
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B D Korant |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of virology Volume: 10 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1972 Oct |
Date Detail:
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Created Date: 1973-01-09 Completed Date: 1973-01-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 751-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell-Free System Cells, Cultured Chymotrypsin / antagonists & inhibitors, pharmacology Electrophoresis, Polyacrylamide Gel Enterovirus B, Human / growth & development, metabolism* Haplorhini Hela Cells / metabolism Humans Ketones / pharmacology Kidney Macaca Molecular Weight Peptides / metabolism Poliovirus / growth & development, metabolism* Precipitin Tests Tritium Trypsin / pharmacology Trypsin Inhibitors / pharmacology Viral Proteins / biosynthesis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Ketones; 0/Peptides; 0/Trypsin Inhibitors; 0/Viral Proteins; 10028-17-8/Tritium; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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