| Cleavage of parathyroid hormone in macrophage endosomes illustrates a novel pathway for intracellular processing of proteins. | |
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MedLine Citation:
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PMID: 2760027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most ligands which are taken up by macrophages are transported to lysosomes where they are degraded to their constituents by a concert of acid hydrolases. This process requires a number of intracellular events which result in the transport of ligands from light density endosomes to the more dense lysosomes. In contrast, our studies have shown that macrophages may process some incoming ligands in endosomes (Diment, S., and Stahl, P. D. (1985) J. Biol. Chem. 260, 15311-15317) and that cathepsin D, an aspartyl protease, is localized in these organelles (Diment, S., Leech, M. S., and Stahl, P. D. (1988) J. Biol. Chem. 263, 6901-6907). Using rabbit alveolar macrophages, which can be subjected to subcellular fractionation, we have traced the intracellular transport and processing of bovine parathyroid hormone (PTH-(1-84]. We present evidence that macrophages internalize PTH-(1-84). Once in endosomes the hormone is cleaved to fragments which include a bioactive peptide, PTH-(1-34), and then the fragments are returned to the extracellular medium, without delivery to lysosomes. The entire cycle from initial binding to release of PTH-(1-34) is achieved within 10-15 min, a time period consistent with findings in vivo. Our data provide evidence for a novel route for processing of an endocytosed ligand. |
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Authors:
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S Diment; K J Martin; P D Stahl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 264 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1989 Aug |
Date Detail:
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Created Date: 1989-09-11 Completed Date: 1989-09-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13403-6 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Washington University School of Medicine, St. Louis Missouri. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Endocytosis Kinetics Macrophages / metabolism* Organoids / metabolism* Parathyroid Hormone / genetics*, metabolism Protein Processing, Post-Translational* Rabbits Subcellular Fractions / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI 20015/AI/NIAID NIH HHS; CA 12858/CA/NCI NIH HHS; DK 09976/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Parathyroid Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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