| Cleavage and oligomerization of gliomedin, a transmembrane collagen required for node of ranvier formation. | |
| | |
MedLine Citation:
|
PMID: 17293346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Gliomedin, which has been implicated as a major player in genesis of the nodes of Ranvier, contains two collagenous domains and an olfactomedin-like domain and belongs to the group of type II transmembrane collagens that includes collagens XIII and XVII and ectodysplasin A. One characteristic of this protein family is that constituent proteins can exist in both transmembrane and soluble forms. Recently, gliomedin expressed at the tips of Schwann cell microvilli was found to bind axonal adhesion molecules neurofascin and NrCAM in interactions essential for Na(+)-channel clustering at the nodes of Ranvier in myelinating peripheral nerves. Interestingly, exogenously added olfactomedin domain was found to have the same effect as intact gliomedin. Here we analyze the tissue form of gliomedin and demonstrate that the molecule not only exists as full-length gliomedin but also as a soluble form shed from the cell surface in a furin-dependent manner. In addition, gliomedin can be further proteolytically processed by bone morphogenetic protein 1/Tolloid-like enzymes, resulting in release of the olfactomedin domain from the collagen domains. Interestingly, the later cleavage induces formation of higher order, insoluble molecular aggregates that may play important roles in Na(+)-channel clustering. |
| | |
Authors:
|
Barbara Maertens; Delana Hopkins; Claus-Werner Franzke; Douglas R Keene; Leena Bruckner-Tuderman; Daniel S Greenspan; Manuel Koch |
Related Documents
:
|
2677626 - Bovine pericardial proteoglycan: biochemical, immunochemical and ultrastructural studies. 10869556 - Bone morphogenetic protein-2 stimulates adipogenic differentiation of mesenchymal precu... 22554196 - The blos1 interacting protein kxd1 is involved in the biogenesis of lysosome-related or... 7890746 - Isolation, characterization, and primary structure of a calcium-binding 63-kda bone pro... 12084836 - The plastidic 2-cysteine peroxiredoxin is a target for a thioredoxin involved in the pr... 10938346 - Specific lipoxygenase isoforms accumulate in distinct regions of soybean pod walls and ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-02-08 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Apr |
Date Detail:
|
Created Date: 2007-04-02 Completed Date: 2007-06-06 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 10647-59 Citation Subset: IM |
Affiliation:
|
Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Autoantigens / metabolism Axons / metabolism Bone Morphogenetic Protein 1 Bone Morphogenetic Proteins / metabolism COS Cells Cell Adhesion Molecules / metabolism Cell Adhesion Molecules, Neuronal / biosynthesis*, metabolism Cercopithecus aethiops Collagen Type XIII / metabolism Ectodysplasins / metabolism Furin / metabolism Humans Metalloendopeptidases / metabolism Metalloproteases / metabolism Mice Microvilli / metabolism Nerve Growth Factors / metabolism Non-Fibrillar Collagens / metabolism Protein Binding Protein Structure, Tertiary Ranvier's Nodes / metabolism* Schwann Cells / metabolism* Sodium Channels / metabolism Tolloid-Like Metalloproteinases |
| Grant Support | |
ID/Acronym/Agency:
|
R01 GM71679/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Autoantigens; 0/Bone Morphogenetic Proteins; 0/Cell Adhesion Molecules; 0/Cell Adhesion Molecules, Neuronal; 0/Collagen Type XIII; 0/Ectodysplasins; 0/Eda protein, mouse; 0/Nerve Growth Factors; 0/Nfasc protein, mouse; 0/Non-Fibrillar Collagens; 0/Sodium Channels; 0/collagen type XVII; 0/gliomedin protein, mouse; EC 3.4.-/Metalloproteases; EC 3.4.-/Tolloid-Like Metalloproteinases; EC 3.4.21.75/Furin; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.19/BMP1 protein, human; EC 3.4.24.19/Bmp1 protein, mouse; EC 3.4.24.19/Bone Morphogenetic Protein 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biochemical and functional characterization of Orai proteins.
Next Document: Regulated binding of adenomatous polyposis coli protein to actin.