Document Detail


Cleavage and gastrulation in the Kuruma shrimp Penaeus (Marsupenaeus) japonicus (Bate): a revised cell lineage and identification of a presumptive germ cell marker.
MedLine Citation:
PMID:  20874712     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous study suggested that mesendoderm (ME) cell arrest occurred at the 64-cell stage and a ring of eight presumptive naupliar mesoderm cells or crown cells surrounded the blastopore in the Kuruma shrimp Penaeus (Marsupenaeus) japonicus. Since this varied from the pattern observed in other penaeoidean shrimp, cleavage and gastrulation was re-examined in P. japonicus using the nucleic acid stain Sytox Green and confocal microscopy. In contrast to the earlier study, cleavage and gastrulation followed the pattern observed in other penaeoidean shrimp. The ME cells arrested at the 32-cell stage, ingressed into the blastocoel, and resumed division after a three cell cycle delay. Nine naupliar mesoderm or crown cells surrounded the blastopore and their descendants invaginated during gastrulation. An intracellular body (ICB) was detected by Sytox Green and SYTO RNASelect staining to be segregated to one ME cell in P. japonicus, as described previously in Penaeus monodon. Staining of the ICB was eliminated by pre-treatment with RNase but not DNase. The ICB was also found in two other penaeoidean shrimp, Penaeus vannamei (Family Penaeidae) and Sicyonia ingentis (Family Sicyoniidae). The results support the hypothesis that the ICB is a germ granule found in the Dendrobranchiata.
Authors:
John B Pawlak; Melony J Sellars; Andrew Wood; Philip L Hertzler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  52     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  677-92     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal compilation © 2010 Japanese Society of Developmental Biologists.
Affiliation:
Central Michigan University, Department of Biology, Mount Pleasant, Michigan 48859, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers*
Cell Cycle
Cell Lineage
Crustacea / cytology*
Gastrulation*
Germ Cells / cytology*
Chemical
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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