| Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage. | |
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MedLine Citation:
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PMID: 2158082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The DNA methylase M.Xbal was isolated from an E. coli recombinant clone. We deduce that the enzyme methylates at the sequence 5'-TCTAGm6A-3'. In combination with the methylation-dependent restriction endonuclease, DpnI (5'-Gm6A/TC-3'), DNA cleavage occurs at the sequence 5'-TCTAGA/TCTAGA-3'. This twelve-base-pair site should occur once every 16,000,000 base pairs in a random sequence of DNA. The exceptional rarity of the M.XbaI/DpnI sequence makes it an ideal candidate for transpositional integration of a unique cleavage site into bacterial genomes. Retrotransposition into mammalian genomes is also an attractive possibility. |
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Authors:
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Y Patel; E Van Cott; G G Wilson; M McClelland |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 18 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-05-16 Completed Date: 1990-05-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1603-7 Citation Subset: IM |
Affiliation:
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California Institute of Biological Research, La Jolla 92037. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviruses, Human
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genetics Bacteriophage lambda / genetics Base Sequence* DNA Restriction Enzymes / metabolism DNA, Viral / metabolism Deoxyribonucleases, Type II Site-Specific / metabolism* Escherichia coli / genetics Methylation Molecular Sequence Data Oligodeoxyribonucleotides* Restriction Mapping Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Oligodeoxyribonucleotides; EC 2.1.1.-/DNA modification methylase XbaI; EC 2.1.1.72/Site-Specific DNA-Methyltransferase (Adenine-Specific); EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/endodeoxyribonuclease DpnI; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 3 Year: 1990 Volume: 18 Issue: 6 First Page: 1603 Last Page: 1607 ID: 330532 PubMed Id: 2158082 |
| Cleavage at the twelve-base-pair sequence 5'-TCTAGATCTAGA-3' using M.Xbal (TCTAGm6A) methylation and DpnI (Gm6A/TC) cleavage. | |
| Y Patel | |
| E Van Cott | |
| G G Wilson | |
| M McClelland | |
| California Institute of Biological Research, La Jolla 92037. |
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