Document Detail


Cleavage of Epstein-Barr virus glycoprotein B is required for full function in cell-cell fusion with both epithelial and B cells.
MedLine Citation:
PMID:  19218203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycoprotein B (gB) homologues within the herpesvirus family display high sequence conservation, and a number of gB homologues contain a cleavage motif R-X-K/R-R recognized by the cellular protease furin. Epstein-Barr virus (EBV) gB contains this motif and cleaved gB is found in EBV virions. To determine the functional significance of this cleavage motif in EBV gB, a deletion mutant (gB Deltafurin) was created lacking the motif. This cleavage mutant was expressed well in cell culture but was not cleaved. Experiments examining gB Deltafurin in a cell-fusion assay revealed that fusion was reduced by 52 % in epithelial and 28 % in B cells when compared with wild-type EBV gB. This decrease in cell-cell fusion is similar to that observed with multiple alphaherpesvirus gB cleavage mutants and supports a conserved function for cleaved gB.
Authors:
Jessica Sorem; Richard Longnecker
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of general virology     Volume:  90     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-16     Completed Date:  2009-04-03     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  591-5     Citation Subset:  IM    
Affiliation:
Department of Microbiology, The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
B-Lymphocytes / virology*
Cell Fusion*
Epithelial Cells / virology*
Gene Deletion
Herpesvirus 4, Human / metabolism,  pathogenicity,  physiology*
Humans
Membrane Fusion
Molecular Sequence Data
Mutagenesis, Site-Directed
Viral Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 AI067048/AI/NIAID NIH HHS; R01 AI067048-03/AI/NIAID NIH HHS; R01 AI076183-07/AI/NIAID NIH HHS; R01 CA117794-04/CA/NCI NIH HHS; T32 AI060523/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Epstein-Barr virus glycoprotein gp110; 0/Viral Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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