| Clearance of rat C-reactive protein in vivo and by perfused liver. | |
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MedLine Citation:
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PMID: 1550988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The clearance in vivo of rat C-reactive protein (CRP) was studied: (i) in the whole animal and (ii) by using a rat liver perfusion system. Rat CRP is a glycosylated serum protein containing a complex-type biantennary carbohydrate structure on each of its five subunits. The half-life of rat asialo CRP was approximately 5 min. More than 75% of the radioactivity associated with rat asialo CRP and asialo alpha 1-acid glycoprotein (AGP) was recovered in the liver. A small amount of radioactivity (0.8%) associated with rat CRP and rat asialo CRP was found in the lungs. Competitive inhibition of the clearance of 125I-labelled rat asialo CRP from the circulation by asialo AGP was dose dependent, and resulted in a corresponding decrease in the recovery of radioactivity associated with rat asialo CRP in the liver. This indicated that asialo AGP and rat asialo CRP were cleared by the hepatic asialoglycoprotein receptor. This observation was confirmed when the clearance of rat asialo CRP was studied using a rat liver perfusion system. Using this system, the clearance of rat asialo CRP and asialo AGP from the perfusate was inhibited by N-acetylgalactosamine, but not by phosphorylcholine, a ligand through which most of the CRP reactions are mediated. This study provides an example of a circulating serum glycoprotein containing a biantennary carbohydrate structure that is cleared by the asialoglycoprotein receptor. |
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Authors:
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C Yang; S Mookerjea; A Nagpurkar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycobiology Volume: 2 ISSN: 0959-6658 ISO Abbreviation: Glycobiology Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-04-30 Completed Date: 1992-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9104124 Medline TA: Glycobiology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 41-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asialoglycoproteins / metabolism C-Reactive Protein / chemistry, metabolism* Carbohydrate Sequence Half-Life Liver / metabolism* Lung / metabolism Male Molecular Sequence Data Orosomucoid / analogs & derivatives, metabolism Perfusion Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoproteins; 0/Orosomucoid; 0/asialoorosomucoid; 9007-41-4/C-Reactive Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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