Document Detail

Clearance of radiation-induced apoptotic lymphocytes: ex vivo studies and an in vitro co-culture model.
MedLine Citation:
PMID:  12236814     Owner:  NLM     Status:  MEDLINE    
Lymphocytes are very sensitive to radiation. Our aim was to test the possibility of detecting apoptosis in lymphocytes as a potential short-term biomarker of ionizing radiation exposure. Our in vitro data confirmed the dose-time-effect relationships involved in radiation-induced apoptosis. The detection of in vivo induction of apoptosis in circulating lymphocytes after exposure of animals to radiation appears to depend critically on the technique used to measure apoptosis. Among the different techniques we investigated, mitochondrial modification was the most appropriate; they allowed establishment of dose-time-effect relationships when animals were observed for 72 h. A model of in vitro phagocytosis of apoptotic lymphocytes by macrophages was developed to mimic clearance of apoptotic cells occurring in vivo. Together, our data show that mitochondrial labeling may make it possible to detect ex vivo radiation-induced apoptosis of lymphocytes before macrophage ingestion occurs. We propose the measurement of apoptosis in lymphocytes as a potential short-term biomarker of ionizing radiation exposure.
M Benderitter; V Durand; C Caux; P Voisin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  158     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-09-18     Completed Date:  2002-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  464-74     Citation Subset:  IM; S    
Institut de Protection et de Sûreté Nucléaire, Département de Protection de la santé de l'Homme et de Dosimétrie, Laboratoire de Dosimétrie Biologique Multiparamétrique, F-92265 Fontenay-aux-Roses Cédex, France.
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MeSH Terms
Apoptosis / radiation effects*
Biological Markers
Coculture Techniques
Dose-Response Relationship, Radiation
Lymphocytes / radiation effects*
Rats, Wistar
Reg. No./Substance:
0/Biological Markers

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