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Clearance of NH2-terminal propeptides of types I and III procollagen is a physiological function of the scavenger receptor in liver endothelial cells.
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MedLine Citation:
PMID:  8294857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to determine the fate of circulating NH2-terminal propeptide of type I procollagen (PINP) in rats. Radiolabeled PINP showed a biphasic serum decay curve after intravenous injection. 79% of the material disappeared from the blood during the initial alpha-phase (t1/2 alpha = 0.6 min), while the remaining 21% was eliminated with a t1/2 beta of 3.3 min. The major site of uptake was the liver, 78, 1, and 21% of its radioactivity being recovered in isolated liver endothelial cells (LEC), Kupffer cells, and parenchymal cells, respectively. In LEC, fluorescently labeled PINP accumulated in small (0.1 microns) peripheral and larger (> 0.1 microns) perinuclear vesicles within 10 min at 37 degrees C after a binding pulse at 4 degrees C. These grew in size with increasing chasing time, reaching a maximum diameter of 1 microns or more after 30 min, and taking the shape of rings that were stained only along their periphery. At chase intervals exceeding 30 min, the size of the vesicles decreased, and after 60 min the stain appeared in smaller, densely stained perinuclearly located vesicles. Degradation of 125I-PINP to free smaller fragments and 125I- was significant after 30 min. Only formaldehyde-treated albumin, acetylated LDL, polyinosinic acid and NH2-terminal propeptide of type III procollagen (PIIINP) competed with PINP for uptake. These findings indicate that clearance of PINP and PIIINP, which are normal waste products generated in large quantities, is a physiological function of the scavenger receptor in LEC.
Authors:
J Melkko; T Hellevik; L Risteli; J Risteli; B Smedsrød
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  179     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-03     Completed Date:  1994-03-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-12     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, University of Oulu, Finland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cattle
Cell Line
Endocytosis
Endothelium / cytology,  metabolism
Humans
Kinetics
Liver / cytology,  metabolism*
Membrane Proteins*
Procollagen / metabolism*
Rats
Receptors, Immunologic / metabolism*,  physiology
Receptors, Lipoprotein*
Receptors, Scavenger
Scavenger Receptors, Class B
Swine
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/Procollagen; 0/Receptors, Immunologic; 0/Receptors, Lipoprotein; 0/Receptors, Scavenger; 0/Scarb1 protein, mouse; 0/Scavenger Receptors, Class B
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 2 Year: 1994
Volume: 179 Issue: 2
First Page: 405 Last Page: 412
ID: 2191385
Publisher Id: 94125021
PubMed Id: 8294857

Clearance of NH2-terminal propeptides of types I and III procollagen is a physiological function of the scavenger receptor in liver endothelial cells


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