Document Detail


Clay ingestion enhances intestinal triacylglycerol hydrolysis and non-esterified fatty acid absorption.
MedLine Citation:
PMID:  19138447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Consumption by animals and humans of earthy materials such as clay is often related to gut pathologies. Our aim was to determine the impact of kaolinite ingestion on glucose and NEFA transport through the intestinal mucosa. The expression of hexose transporters (Na/glucose co-transporter 1 (SGLT1), GLUT2, GLUT5) and of proteins involved in NEFA absorption (fatty acid transporter/cluster of differentiation 36 (FAT/CD36), fatty acid transport protein 4 (FATP4) and liver fatty acid binding protein (L-FABP)) was measured (1) in rats whose jejunum was perfused with a solution of kaolinite, and (2) in rats who ate spontaneously kaolinite pellets during 7 and 28 d. Also, we determined TAG and glucose absorption in the kaolinite-perfused group, and pancreatic lipase activity, gastric emptying and intestinal transit in rats orally administered with kaolinite. Glucose absorption was not affected by kaolinite perfusion or ingestion. However, kaolinite induced a significant increase in intestinal TAG hydrolysis and NEFA absorption. The cytoplasmic expression of L-FABP and FATP4 also increased due to kaolinite ingestion. NEFA may enter the enterocytes via endocytosis mainly since expression of NEFA transporters in the brush-border membrane was not affected by kaolinite. After uptake, rapid binding of NEFA by L-FABP and FATP4 could act as an intracellular NEFA buffer to prevent NEFA efflux. Increased TAG hydrolysis and NEFA absorption may be due to the adsorption properties of clay and also because kaolinite ingestion caused a slowing down of gastric emptying and intestinal transit.
Authors:
Caroline Habold; François Reichardt; Yvon Le Maho; Fabielle Angel; Nicole Liewig; Jean-Hervé Lignot; Hugues Oudart
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-13
Journal Detail:
Title:  The British journal of nutrition     Volume:  102     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-09     Completed Date:  2009-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  249-57     Citation Subset:  IM    
Affiliation:
Institut Pluridisciplinaire Hubert Curien, UMR 7178 CNRS-ULP, 23 Rue du Loess, BP28, 67037 Strasbourg Cedex 2, France. caroline.habold@c-strasbourg.fr
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animals
Antidiarrheals / administration & dosage*
Fatty Acid Transport Proteins / genetics
Fatty Acid-Binding Proteins / genetics
Fatty Acids, Nonesterified / metabolism*
Gastric Emptying / physiology
Gastrointestinal Transit
Glucose / metabolism
Glucose Transporter Type 5 / genetics
Hydrolysis
Intestinal Absorption / physiology*
Intestines / metabolism*
Kaolin / administration & dosage*
Lipase / analysis
Male
RNA, Messenger / analysis
Rats
Rats, Wistar
Sodium-Glucose Transporter 1 / genetics
Triglycerides / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Antidiarrheals; 0/FATP4 protein, rat; 0/Fatty Acid Transport Proteins; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids, Nonesterified; 0/Glucose Transporter Type 5; 0/RNA, Messenger; 0/Sodium-Glucose Transporter 1; 0/Triglycerides; 1332-58-7/Kaolin; 50-99-7/Glucose; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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