| Claudin 11 deficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis. | |
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MedLine Citation:
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PMID: 19741204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tissue integrity relies on barriers formed between epithelial cells. In the testis, the barrier is formed at the initiation of puberty by a tight junction complex between adjacent Sertoli cells, thereby defining an adluminal compartment where meiosis and spermiogenesis occur. Claudin 11 is an obligatory protein for tight junction formation and barrier integrity in the testis. It is expressed by Sertoli cells, and spermatogenesis does not proceed beyond meiosis in its absence, resulting in male sterility. Sertoli cell maturation--arrest of proliferation and expression of proteins to support germ cell development--parallels tight junction assembly; however, the pathophysiology underlying the loss of tight junctions in the mature testis remains largely undefined. Here, using immunohistochemistry and microarrays we demonstrate that adult Cldn11(-/-) mouse Sertoli cells can proliferate while maintaining expression of mature markers. Sertoli cells detach from the basement membrane, acquire a fibroblast cell shape, are eliminated through the lumen together with apoptotic germ cells, and are found in epididymis. These changes are associated with tight junction regulation as well as actin-related and cell cycle gene expression. Thus, Cldn11(-/-) Sertoli cells exhibit a unique phenotype whereby loss of tight junction integrity results in loss of the epithelial phenotype. |
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Authors:
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S Mazaud-Guittot; E Meugnier; S Pesenti; X Wu; H Vidal; A Gow; B Le Magueresse-Battistoni |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-09-09 |
Journal Detail:
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Title: Biology of reproduction Volume: 82 ISSN: 1529-7268 ISO Abbreviation: Biol. Reprod. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-21 Completed Date: 2010-03-30 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 202-13 Citation Subset: IM |
Affiliation:
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INSERM, U870, Oullins, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Apoptosis Blood-Testis Barrier* Cell Division Cell Polarity Cell Transdifferentiation* Gene Expression Profiling Male Mice Mice, Inbred C57BL Nerve Tissue Proteins / metabolism* Oligonucleotide Array Sequence Analysis Phenotype Sertoli Cells / cytology, physiology* Spermatogenesis* Tight Junctions / physiology |
| Grant Support | |
ID/Acronym/Agency:
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DC006262/DC/NIDCD NIH HHS; R01 DC006262-05/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Cldn11 protein, mouse; 0/Nerve Tissue Proteins |
| Comments/Corrections | |
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