Document Detail


Claudin 11 deficiency in mice results in loss of the Sertoli cell epithelial phenotype in the testis.
MedLine Citation:
PMID:  19741204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue integrity relies on barriers formed between epithelial cells. In the testis, the barrier is formed at the initiation of puberty by a tight junction complex between adjacent Sertoli cells, thereby defining an adluminal compartment where meiosis and spermiogenesis occur. Claudin 11 is an obligatory protein for tight junction formation and barrier integrity in the testis. It is expressed by Sertoli cells, and spermatogenesis does not proceed beyond meiosis in its absence, resulting in male sterility. Sertoli cell maturation--arrest of proliferation and expression of proteins to support germ cell development--parallels tight junction assembly; however, the pathophysiology underlying the loss of tight junctions in the mature testis remains largely undefined. Here, using immunohistochemistry and microarrays we demonstrate that adult Cldn11(-/-) mouse Sertoli cells can proliferate while maintaining expression of mature markers. Sertoli cells detach from the basement membrane, acquire a fibroblast cell shape, are eliminated through the lumen together with apoptotic germ cells, and are found in epididymis. These changes are associated with tight junction regulation as well as actin-related and cell cycle gene expression. Thus, Cldn11(-/-) Sertoli cells exhibit a unique phenotype whereby loss of tight junction integrity results in loss of the epithelial phenotype.
Authors:
S Mazaud-Guittot; E Meugnier; S Pesenti; X Wu; H Vidal; A Gow; B Le Magueresse-Battistoni
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-09
Journal Detail:
Title:  Biology of reproduction     Volume:  82     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-03-30     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  202-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Apoptosis
Blood-Testis Barrier*
Cell Division
Cell Polarity
Cell Transdifferentiation*
Claudins
Gene Expression Profiling
Male
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / metabolism*
Oligonucleotide Array Sequence Analysis
Phenotype
Sertoli Cells / cytology,  physiology*
Spermatogenesis*
Tight Junctions / physiology
Grant Support
ID/Acronym/Agency:
DC006262/DC/NIDCD NIH HHS; R01 DC006262/DC/NIDCD NIH HHS; R01 DC006262-05/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Claudins; 0/Cldn11 protein, mouse; 0/Nerve Tissue Proteins
Comments/Corrections

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