|Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG.|
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|PMID: 22891263 Owner: NLM Status: MEDLINE|
|Clathrin depletion by ribonucleic acid interference (RNAi) impairs mitotic spindle stability and cytokinesis. Depletion of several clathrin-associated proteins affects centrosome integrity, suggesting a further cell cycle function for clathrin. In this paper, we report that RNAi depletion of CHC17 (clathrin heavy chain 17) clathrin, but not the CHC22 clathrin isoform, induced centrosome amplification and multipolar spindles. To stage clathrin function within the cell cycle, a cell line expressing SNAP-tagged clathrin light chains was generated. Acute clathrin inactivation by chemical dimerization of the SNAP-tag during S phase caused reduction of both clathrin and ch-TOG (colonic, hepatic tumor overexpressed gene) at metaphase centrosomes, which became fragmented. This was phenocopied by treatment with Aurora A kinase inhibitor, suggesting a centrosomal role for the Aurora A-dependent complex of clathrin, ch-TOG, and TACC3 (transforming acidic coiled-coil protein 3). Clathrin inactivation in S phase also reduced total cellular levels of ch-TOG by metaphase. Live-cell imaging showed dynamic clathrin recruitment during centrosome maturation. Therefore, we propose that clathrin promotes centrosome maturation by stabilizing the microtubule-binding protein ch-TOG, defining a novel role for the clathrin-ch-TOG-TACC3 complex.|
|Amy B Foraker; Stéphane M Camus; Timothy M Evans; Sophia R Majeed; Chih-Ying Chen; Sabrina B Taner; Ivan R Corrêa; Stephen J Doxsey; Frances M Brodsky|
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|Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-13|
|Title: The Journal of cell biology Volume: 198 ISSN: 1540-8140 ISO Abbreviation: J. Cell Biol. Publication Date: 2012 Aug|
|Created Date: 2012-08-21 Completed Date: 2012-11-05 Revised Date: 2013-07-15|
Medline Journal Info:
|Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: United States|
|Languages: eng Pagination: 591-605 Citation Subset: IM|
|Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94143, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Clathrin / genetics, physiology*
Clathrin Heavy Chains / antagonists & inhibitors, genetics, metabolism*
Microtubule-Associated Proteins / metabolism*
Mitosis / physiology*
RNA Stability / genetics*
RNA, Small Interfering / genetics
|GM038093/GM/NIGMS NIH HHS; GM051994/GM/NIGMS NIH HHS; R01 GM038093/GM/NIGMS NIH HHS; T32 CA09043/CA/NCI NIH HHS|
|0/CHC17 protein, human; 0/CLTCL1 protein, human; 0/Clathrin; 0/Microtubule-Associated Proteins; 0/RNA, Small Interfering; 0/ch-TOG protein, human; 114899-12-6/Clathrin Heavy Chains|
|Nat Rev Mol Cell Biol. 2012 Oct;13(10):606
Journal ID (nlm-ta): J Cell Biol
Journal ID (iso-abbrev): J. Cell Biol
Journal ID (hwp): jcb
Publisher: The Rockefeller University Press
© 2012 Foraker et al.
Received Day: 5 Month: 12 Year: 2011
Accepted Day: 12 Month: 7 Year: 2012
Print publication date: Day: 20 Month: 8 Year: 2012
Volume: 198 Issue: 4
First Page: 591 Last Page: 605
PubMed Id: 22891263
Publisher Id: 201205116
|Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG Alternate Title:Clathrin function at the centrosome|
|Amy B. Foraker1234|
|Stéphane M. Camus1234|
|Timothy M. Evans1234|
|Sophia R. Majeed1234|
|Sabrina B. Taner1234|
|Ivan R. Corrêa, Jr5|
|Stephen J. Doxsey6|
|Frances M. Brodsky1234|
1Department of Bioengineering and Therapeutic
Sciences, 2Department of
Pharmaceutical Chemistry, 3Department
of Microbiology and Immunology, and
4The G.W. Hooper Foundation, University of
California San Francisco, San Francisco, CA 94143
5New England Biolabs, Inc., Ipswich, MA
6Program in Molecular Medicine, University of
Massachusetts Medical School, Worcester, MA 01605
|Correspondence: Correspondence to Frances M. Brodsky:
Clathrin-mediated pathways are required for normal mitotic progression and cytokinesis in vertebrate and slime mold cells (Niswonger and O’Halloran, 1997; Feng et al., 2002; Royle et al., 2005; Schweitzer et al., 2005; Boucrot and Kirchhausen, 2007; Lin et al., 2010; Royle, 2012). Multinucleated cells and abscission defects are generated by clathrin disruption through RNAi, genetic deletion, and expression of dominant-negative fragments, which have been analyzed in the context of accumulated rounds of mitosis. Clathrin’s roles in the cell cycle have been ascribed to mitotic spindle stabilization (Royle et al., 2005; Royle and Lagnado, 2006; Fu et al., 2010; Lin et al., 2010; Booth et al., 2011) as well as to endosomal membrane traffic needed for cell expansion and abscission (Niswonger and O’Halloran, 1997; Feng et al., 2002; Thompson et al., 2002; Schweitzer et al., 2005; Boucrot and Kirchhausen, 2007). RNAi studies also implicate several clathrin-associated proteins in centrosome formation (Thompson et al., 2004; Lehtonen et al., 2008; Liu and Zheng, 2009; Shimizu et al., 2009). Disruption of centrosome integrity can induce multinucleation and abscission defects, raising the questions addressed here of whether clathrin itself is involved in centrosome function and, if so, which mitotic phenotypes result from disruption of which clathrin functions during the cell cycle. Here, we develop a new strategy for acute inactivation of clathrin within the time frame of the cell cycle to define distinct roles for CHC17 (clathrin heavy chain 17) and its isoform CHC22 in cell division and establish how interference with these pathways induces specific mitotic defects.
Clathrin is a cytosolic protein with a three-legged triskelion shape generated by trimerization of clathrin heavy chain (CHC) subunits. In interphase, triskelia assemble into lattices that coat intracellular membranes by interaction with adaptor molecules (Brodsky et al., 2001). There are two CHC isoforms in vertebrates, CHC17 and CHC22, that share 85% sequence identity (Wakeham et al., 2005). CHC17 clathrin has a well-characterized role in endocytosis and sorting at the trans-Golgi network and endosomes. CHC17 also localizes to the mitotic spindle (Okamoto et al., 2000; Royle et al., 2005; Esk et al., 2010). CHC22 is most highly expressed in muscle, where it is involved in GLUT4 glucose transporter traffic (Vassilopoulos et al., 2009). In all cells, CHC22 functions in endosomal sorting at a distinct step from CHC17 and is not observed on the mitotic spindle under normal conditions (Esk et al., 2010). The CHC17 triskelion binds light chain subunits, which do not associate with the cellular form of CHC22 (Liu et al., 2001). Vertebrates have two exchangeable clathrin light chains (CLCs), LCa and LCb (Wakeham et al., 2005), that are expressed as nonneuronal or neuronal splice variants.
Studies to date make a compelling case for CHC17 clathrin playing a role in membrane traffic needed for cell division and a separate role in contributing to mitotic spindle stability. Although the status of clathrin-mediated endocytosis during early mitosis is debated (Sager et al., 1984; Schweitzer et al., 2005; Boucrot and Kirchhausen, 2007), it is agreed that from anaphase onset to cytokinesis exit, CHC17 clathrin functions in endosomal membrane-trafficking events that stabilize the equatorial cleavage furrow (Niswonger and O’Halloran, 1997; Gerald et al., 2001; Feng et al., 2002; Warner et al., 2006), provide membrane for precytokinetic expansion (Boucrot and Kirchhausen, 2007), and contribute to midbody abscission (Thompson et al., 2002; Schweitzer et al., 2005; Prekeris and Gould, 2008; Joshi et al., 2010). Clathrin-coated vesicles at spindle poles have also been implicated in postmitotic Golgi reassembly (Radulescu and Shields, 2012). Recent studies of CHC17 indicate a direct role in stabilization of spindle microtubules through formation of a complex with TACC3 (transforming acidic coiled-coil protein 3) and ch-TOG (colonic, hepatic tumor overexpressed gene) (Fu et al., 2010; Hubner et al., 2010; Lin et al., 2010; Booth et al., 2011). These are nonmotor proteins that form a complex at minus and plus ends of microtubules and function in centrosome organization and stabilizing mitotic spindles (Gergely et al., 2000, 2003; Cassimeris and Morabito, 2004; Peset and Vernos, 2008). RNAi depletion of proteins known to bind CHC17, including the ARH (autosomal recessive hypercholesterolemia) adaptor (Lehtonen et al., 2008), epsin1 (Liu and Zheng, 2009), and cyclin G–associated kinase (GAK; Shimizu et al., 2009), disrupts centrosome maturation and integrity, suggesting CHC17 plays a role in centrosome function that could directly or indirectly influence later mitotic events.
In this study, we first use RNAi to deplete either CHC isoform to examine their comparative influence on centrosomes and cell division. We then selectively target CHC17 for acute inactivation in S phase or later in mitosis by transfecting cells with SNAP-tagged LCa that can be chemically cross-linked in situ (Lemercier et al., 2007). This strategy identified a novel function for CHC17 in centrosome maturation between S phase and the G2/M transition that, when disrupted, caused loss of centrosome integrity in metaphase and later mitotic defects. CHC22 function was not required for centrosome integrity, but its depletion caused multinucleation. Thus, multiple stages of cell division are influenced by specific clathrin functions.
A possible role for clathrin in centrosome maturation has been suggested by depletion of clathrin-interacting proteins (Lehtonen et al., 2008; Liu and Zheng, 2009; Shimizu et al., 2009). To investigate this, we analyzed centrosome morphology and number after RNAi-mediated depletion of CHC17, its associated CLCs (LCa and LCb), or the CHC22 isoform. These studies were performed in HeLa cells stably expressing GFP–α-tubulin (HeLa-GFP–α-tubulin) to visualize microtubule networks as well as immunolabeled centrosome proteins. After 72 h of treatment, siRNA against CHC17 reduced both CHC17 and CLCs by ≥50%, and codepletion of both CLCs caused partial reduction of CHC17 (Figs. 1 A and S1 B), consistent with known interdependence of clathrin subunits for their stability (Brodsky, 1985b; Ybe et al., 2007). The highest siRNA concentration (20 nM) targeting CLCs also led to an ∼50% reduction in CHC22, not unexpected because CHC22 expression indirectly depends on CHC17 pathways for its stability (Esk et al., 2010). CHC22 depletion resulted in ≥50% reduction of CHC22, with no significant effect on other clathrin proteins, as previously observed (Vassilopoulos et al., 2009; Esk et al., 2010). Effects of siRNAs were quantified in different HeLa clones compared with nonsilencing treatments (Fig. S1).
Centrosomes were visualized by immunofluorescence (IF) to detect centrosome proteins γ-tubulin (Fig. 1) and pericentrin-2 (PCNT2; Fig. 2) in prometaphase and metaphase cells after siRNA depletion (72 h) of clathrin components. Defects were obvious using both markers. Depletion of CHC17 or CLCs doubled the percentage of cells with either amplified centrosomes or multipolar spindles (more than two of each), whereas codepletion of CHC17 and CLCs quadrupled the defective population (Fig. 1, B and D). CHC17- and/or CLC-depleted cells also displayed signs of centrosome fragmentation (Fig. 2) as well as reduced or diffuse fluorescence intensity of centrosome proteins at spindle poles (Figs. 1 B and 2). Levels of γ-tubulin or PCNT2 in lysates of clathrin-depleted cells (Fig. S1 B and not depicted) were unaffected. Therefore, observed changes reflect altered subcellular localization of these centrosome components, not their altered protein stability. CHC22 depletion did not affect centrosome number or spindle nucleation (Fig. 1, C and D). Thus, CHC17 and associated CLCs, but not CHC22, mediate pathways that affect centrosome number, integrity, and subsequent spindle nucleation. Coincidence of multipolar spindles with centrosome integrity defects (Fig. 1 D) correlates with known pathways of spindle nucleation in mammalian cells (Lingle and Salisbury, 1999).
Centrosome numbers and morphology are determined by centriole duplication and centrosome maturation, which occur during G1/S- and G2/M-phase transitions, respectively (Sluder and Rieder, 1985; Azimzadeh and Bornens, 2007). HeLa cells stably expressing the centriolar protein centrin-2 (Salisbury et al., 2002) tagged with GFP (HeLa–centrin-2–GFP) were analyzed to determine whether effects of clathrin depletion on centrosomes could be explained by an effect on duplication. A slight, but not statistically significant, increase in cells with more than the expected four centrioles produced by normal duplication was observed upon depletion of CHC17 clathrin subunits (Fig. 3) compared with treatment with nonsilencing siRNA or siRNA-targeting CHC22. Levels of PCNT2 (not depicted), which can affect centriole amplification (Pihan et al., 2001) and levels of endogenous centrin-2 (Fig. 3 A), were not significantly altered upon any siRNA treatment. These observations suggest that CHC17 does not play a direct role in centriole duplication but may affect centrosome number through another pathway.
A block in cytokinesis can also cause centrosome defects (Lingle et al., 2005). Indeed, depletion of CHC17 resulted in a trend toward multinucleation after 48 or 72 h of siRNA treatment (Figs. 4 and S1 [A and B]), as previously reported (Royle et al., 2005). Depletion of CHC22, which did not cause centrosomal defects (Fig. 1), produced similar phenotypes of multinucleation, suggesting a distinct role for CHC22 in the cell cycle. Loss of either CHC did not produce the significant level of multinucleation seen after depletion of γ-tubulin, an established participant in cytokinesis (Fig. 4 B), and CLC depletion by itself had little effect on multinucleation. The latter observation is consistent with recent studies showing that, although CLC contributes to CHC17 stability and assembly control, CLC depletion does not mimic CHC17 depletion but mainly affects CLC regulation of actin associated with CHC17-coated membrane (Chen and Brodsky, 2005; Wilbur et al., 2008; Bonazzi et al., 2011).
The most significant phenotype observed in these siRNA depletion studies was amplification of centrosome number by jointly targeting CHC17 and CLCs (Fig. 1). In addition, alteration of centrosome integrity and morphology and spindle multipolarity was observed (Fig. 2). Localization of CHC17 clathrin to centrosomes was not obvious in these samples (Figs. 1, 2, and 3) fixed by PFA. But, in samples fixed in methanol, CHC17 detected by antibodies to either the heavy or light chain subunits was observed to colocalize with the centrosomal markers PCNT2 and γ-tubulin in nontransfected HeLa cells (Fig. 5 A), suggesting the possibility that CHC17 functions directly at the centrosome.
Although siRNA-based depletion indicates cell cycle–related clathrin functions, the relationship of these functions to phenotype is not possible to establish because two to three rounds of cell division occur during the period over which siRNA treatments are performed. Therefore, to investigate further how CHC17 clathrin might influence centrosomes and address whether cell cycle perturbations caused by clathrin depletion could arise from centrosomal defects, we developed an acute inactivation strategy based on the SNAP-tag technology (Gautier et al., 2008) to target CHC17 clathrin specifically. The SNAP-tag is a 20-kD protein derived from the human DNA repair enzyme O6-alkylguanine–DNA alkyltransferase (Juillerat et al., 2003), which covalently binds O6-benzylguanine (BG; Keppler et al., 2004) and its derivatives, many of which are cell permeable. Fluorescent BG derivatives label intracellular SNAP fusion proteins in live cells, whereas BG–glutaric acid (GLA)–BG covalently cross-links two proximal SNAP-tagged proteins when added to live cells (Lemercier et al., 2007). To use SNAP-tag–mediated cross-linking for acute clathrin inactivation, we transfected HeLa cells with a construct encoding ubiquitous (nonneuronal) LCa (uLCa) with the SNAP-tag at the N terminus (SNAP-uLCa, a 57-kD fusion protein) and selected a stable cell line, clone 3.3. The uLCa is the dominant isoform of CLCs in HeLa cells. CLCs compete with each other for CHC17 binding (Brodsky et al., 1987), so transfected CLCs readily replace endogenous CLCs (Acton et al., 1993), when expressed at higher levels, and excess CLCs are rapidly degraded (Brodsky, 1985b). Furthermore, CLCs do not bind to intracellular CHC22 (Liu et al., 2001), thus cross-linking SNAP-uLCa targets CHC17 but not CHC22 for inactivation.
To establish its normal behavior and potential to cross-link associated CHC17, the SNAP-tagged uLCa in clone 3.3 was shown to bind CHC17 by coimmunoprecipitation (co-IP; Fig. S2 A). Additionally, SNAP-uLCa, labeled with the BG-derivative SNAP-TMR-Star, colocalized with endogenous CHC17 in interphase cells and on the mitotic spindle (Fig. S2 B) in PFA-fixed cells. In methanol-fixed cells, the SNAP-uLCa colocalized clearly with centrosomal markers in interphase and metaphase (Fig. 5 B). The latter colocalization was specific, as endosomal markers transferrin receptor (TfR) or EEA1 (early endosomal antigen 1) did not localize to centrosomes (Fig. 5 C). When incubated with BG-GLA-BG, dimerization of the SNAP-tagged uLCa was readily detected (Fig. 6 A), and variation of time and concentration revealed that a 5–10-µM treatment for 2 h was sufficient for optimal cross-linking. After treatment with 5 µM BG-GLA-BG for 2 h, clone 3.3 cells were tested for uptake of fluorescent transferrin over 10 min and 1 h (Fig. 6, B and C). Compared with mock-treated cells, in which transferrin was observed in intracellular vesicles within 10 min, transferrin remained largely at the plasma membrane in BG-GLA-BG–treated cells, indicating disruption of clathrin-mediated endocytosis. After 1 h of transferrin uptake, mock-treated cells were almost completely clear of subcellular fluorescent transferrin, a sign of efficient recycling and some degradation. In contrast, cells treated with BG-GLA-BG showed fluorescent transferrin still present in vesicles labeled by IF for CHC17 and EEA1 (Fig. 6, B and C), reflecting diminished uptake of fluorescent transferrin and a block in its endosomal sorting or recycling. It is well documented that after blocking clathrin-mediated uptake, transferrin can be internalized by nonclathrin-mediated pathways and is then detected with clathrin in sorting endosomes (Damke et al., 1994; Bennett et al., 2001). Impaired uptake of TfR after exposure of clone 3.3 to cross-linker was further demonstrated using a cell surface biotinylation assay. Clone 3.3 cells that were treated with or without BG-GLA-BG were labeled with reducible biotin on ice and then incubated at 37°C (5–15 min) to induce internalization (Fig. 6, D and E). After stripping the remaining surface biotin by reduction, cell lysates were prepared, and internalized TfR was detected by immunoblotting streptavidin (SA)-bound proteins. Whereas mock-treated cells showed an increase in internal TfR (SA bound), BG-GLA-BG–treated cells did not. Additionally, surface levels of TfR on the BG-GLA-BG–treated cells were lower at the start of the internalization experiment, reflecting endosomal trapping of TfR that was occurring during the 2 h of cross-linker treatment before biotinylation. Together, analysis of transferrin and TfR traffic showed that BG-GLA-BG treatment of clone 3.3 is effective for acute inactivation of clathrin function in receptor-mediated endocytosis and shares phenotypic effects with other acute clathrin inactivation methods (Moskowitz et al., 2003; Deborde et al., 2008).
To acutely inactivate CHC17 function at specific points during the cell cycle, HeLa-SNAP-uLCa cells were enriched to S phase by double thymidine block (T/T; Fig. 7 A). Cell cycle synchronization was confirmed by propidium iodide labeling of DNA and flow cytometry, which revealed that cells were enriched to the G2/M phase between 7 and 8 h after release from the second thymidine block. Synchronized cells were then treated with BG-GLA-BG immediately after the block was released just before mitotic entry (T0; Fig. 7 A) or treated with BG-GLA-BG 7 h after T/T at the time of metaphase onset (T7; Fig. 7 A) or mock treated at these times. These were 2-h treatments and therefore extended 0–2 and 7–9 h after T/T for T0 and T7, respectively. Cell lysates were prepared during the post-T/T period, and comparable levels of covalent dimers of SNAP-uLCa were detected between 2 and 12 h after T0 treatment and at 12 h after T7 treatment (Fig. 7 B). Thus, SNAP-tag–mediated cross-linking is effective in synchronized cells. We further examined the levels for CHC and CLC isoforms in cells treated at T0 and harvested 2–12 h after T/T, and these were unchanged when comparing BG-GLA-BG and mock treatments (Fig. S3, A and B).
The T0 treatment with BG-GLA-BG made it possible to assess whether the centrosome amplification and multipolar spindles that occur upon CHC17 depletion (Figs. 1 and 2) are an immediate or long-term effect of losing clathrin function. Examination of cells 2 h after BG-GLA-BG treatment (4 h, after T0) showed no obvious change in centrosome morphology (Fig. 7 C). However, by early mitosis (8 h, after T0), fragmented centrosomes were observed (Fig. 7 D) as well as multipolar spindles (Fig. S3 C). Quantification of early mitotic cells with fragmented centrosomes confirmed the significance of this observation (Fig. 7 E). Analysis of γ-tubulin distribution relative to the central point of spindle pole–associated centrosomes (Fig. 7 F) revealed significant dispersion of the γ-tubulin signal in BG-GLA-BG–treated prometaphase cells. In contrast to clathrin depletion by siRNA, acute CHC17 inactivation produced only marginal increases in multinucleated cells after T0 and T7 BG-GLA-BG treatments when compared with mock treatments (Fig. S3 D). Thus, although acute inactivation of CHC17 at T0 during S phase affects centrosome integrity during early mitosis, a significant multinuclear phenotype is not an immediate consequence of clathrin inactivation during this period. Notably, multinucleation was observed even less after clathrin inactivation later in the cell cycle at T7, suggesting that the multinucleation that occurs after siRNA depletion of clathrin (Fig. 4) reflects compounded effects over multiple cell cycles.
Aurora A protein levels and kinase activity peak at the onset of G2/M, and inhibition of its activity is documented to prevent centrosome separation, centrosome maturation, and bipolar spindle formation (Hannak et al., 2001; Berdnik and Knoblich, 2002; Marumoto et al., 2003; Mori et al., 2007; Sardon et al., 2008). The centrosome phenotype induced by treating clone 3.3 cells in S phase with BG-GLA-BG was comparable with defects caused by treatment of cells with the Aurora A kinase inhibitor MLN8054, with respect to frequency of centrosome fragmentation and changes in γ-tubulin distribution (Fig. 7, D–F). Aurora A kinase activity is also required for formation of a complex between CHC17 clathrin, TACC3, and ch-TOG at the mitotic spindle, which stabilizes kinetochore microtubules (Fu et al., 2010; Lin et al., 2010; Booth et al., 2011; Cheeseman et al., 2011). During mitosis, both TACC3 and ch-TOG proteins are largely concentrated at centrosomes (Charrasse et al., 1998; Gergely et al., 2000) and spindles and are established to function in centrosome maturation and spindle pole formation as well as spindle stability (Gergely et al., 2003; Cassimeris and Morabito, 2004). Therefore, we investigated whether TACC3 and ch-TOG were present with clathrin at the centrosome in interphase cells (Fig. 8) and found that all three components were colocalized. Colocalization was not dramatically affected after 2 h of BG-GLA-BG treatment, though the CLC signal at the centrosome was reduced by cross-linking (Fig. 8, A and B). However, by 8 h into the cell cycle (6 h after cross-linking in S phase), ch-TOG protein levels were reduced about twofold compared with mock-treated cells (Fig. 8, C and E). As cells progress to mitosis, both TACC3 and ch-TOG proteins are known to steadily increase and peak at mitotic onset (Gergely et al., 2003). This increase was not observed for ch-TOG after clathrin inactivation, whereas TACC3 levels were not significantly affected (Fig. 8, C–E).
Consistent with a reduction in overall ch-TOG levels, IF analysis showed that ch-TOG was partially reduced at metaphase centrosomes 6 h after clathrin cross-linking in S phase (Fig. 9). Clathrin itself was also reduced at the centrosome 2 h after and 6 h after S-phase treatment with cross-linker (Figs. 7 C and 9 A), suggesting that clathrin association with the centrosome must be dynamic, as confirmed by live imaging studies described in the next section. Again, the morphological effects of acute clathrin inactivation in S phase were mimicked by treatment of cells with Aurora A kinase inhibitor MLN8054, which caused a reduction of clathrin and ch-TOG at the early mitotic centrosome (Fig. 9). This further suggests that formation of the Aurora A–dependent clathrin–TACC3–ch-TOG complex contributes to centrosomal localization of these components. FACS analysis indicated that the timing of cell cycle entry was not affected by clathrin inactivation in S phase (Fig. S4), though effects on centrosomes and ch-TOG levels were observed early in mitosis (Figs. 7–9). This suggests that clathrin inactivation affects centrosome integrity just after G2/M onset, a period in which centrosome maturation occurs.
An advantage to the SNAP-tag is that it can be visualized in live cells using SNAP-TMR-Star. Clone 3.3 was transiently transfected to express GFP-pericentrin to perform comparative live-cell imaging of clathrin and pericentrin in the vicinity of the centrosome. Pericentrin is a constant resident of interphase and mitotic centrosomes in somatic cells and is known to be a key player in centrosome and spindle organization (Doxsey et al., 1994; Dictenberg et al., 1998). Additionally, pericentrin has been shown to traffic to the centrosome via pericentriolar satellite vesicles along microtubules (Young et al., 2000). Time-lapse confocal microscopy (at 37°C with 5% CO2) was performed for live-cell imaging of CHC17 clathrin with SNAP-uLCa bound by SNAP-TMR-Star and the expressed GFP-pericentrin (48 h). Clathrin was constitutively colocalized with GFP-pericentrin at interphase and mitotic centrosomes (Fig. 10 A). In addition, vesicle-like structures that were positive for both SNAP-TMR-Star–labeled clathrin and GFP-pericentrin were observed in the vicinity of centrosomes (Fig. 10 [A and B] and Video 1), with the distribution of previously described pericentriolar satellites. By magnification of events at a late-interphase centrosome and performing time-lapse imaging with shorter scanning intervals, it was possible to visualize satellite structures colabeled for GFP-pericentrin and clathrin that were moving toward centrosomes (Fig. 10 C and Video 2). These time-lapse images indicate that clathrin localizes to centrosomes using a dynamic pathway that is shared by pericentrin and that their colocalization persists throughout the cell cycle. The data further suggest that clathrin recruitment to the centrosome coincides with a known centrosome maturation pathway (Telzer and Rosenbaum, 1979; Kuriyama and Borisy, 1981; Nigg and Raff, 2009).
In this study, we identify a novel role for CHC17, and not CHC22 clathrin, in centrosome maturation. Clathrin isoform roles were distinguished and defined by a combination of RNAi depletion of clathrin isoforms and acute inactivation of CHC17 using chemical cross-linking. Inactivation of CHC17 in S phase resulted in centrosome fragmentation during the ensuing early mitosis as well as an increase in amplified centrosomes and multipolar spindles. These observations recapitulated centrosome amplification induced by RNAi depletion of CHC17 and indicated direct participation of CHC17 in centrosome maturation. This defines a new cell cycle role for CHC17 in addition to its recent implications in mitotic spindle stability (Fu et al., 2010; Lin et al., 2010; Booth et al., 2011) and in endosomal transport during cytokinesis (Thompson et al., 2002; Schweitzer et al., 2005; Prekeris and Gould, 2008; Joshi et al., 2010). Furthermore, these observations explain previous reports that perturbation of clathrin-associated proteins caused centrosomal defects (Thompson et al., 2004; Lehtonen et al., 2008; Liu and Zheng, 2009; Shimizu et al., 2009). In contrast, depletion of the CHC22 clathrin isoform by RNAi did not induce centrosome amplification, though its depletion did generate a multinuclear phenotype comparable with that observed for CHC17 depletion, suggesting that CHC22 contributes to cell division by a distinct mechanism.
To investigate clathrin function within the time frame of a single cell cycle and thereby address roles for clathrin at specific stages, we used a new approach to acute inactivation of clathrin function. In our hands, the SNAP-tag modification we adopted (Lemercier et al., 2007) was more effective at clathrin inactivation than the FK506-binding protein modification (Moskowitz et al., 2003), Killer red modification (Bulina et al., 2006), or the CALI (chromophore-assisted light inactivation) modification (Heerssen et al., 2008), the latter being plagued by background binding of the light-sensitive Lumio compound (unpublished data). The SNAP-tag was also useful for binding fluorescent dye as well as cross-linker (Juillerat et al., 2003; Lemercier et al., 2007). The cross-linking approach to inactivation has the advantage of targeting clathrin itself, rather than interfering with clathrin–adaptor interactions like the recently developed pitstop chemical inhibitors of clathrin function (von Kleist et al., 2011). Although useful, the pitstops may be limited to affecting trafficking functions of clathrin in cells and may not probe clathrin interactions that do not depend on binding the clathrin box of adaptors that they target.
Acute inactivation of CHC17 clathrin in S phase using the BG-GLA-BG cross-linker bound to SNAP-tagged CLC produced a fragmented centrosome phenotype in early mitosis but had no effect on cell cycle entry. Cross-linker treatment in S phase also reduced the presence of clathrin at mitotic centrosomes and reduced whole-cell levels of ch-TOG as well as its levels at mitotic centrosomes. These observations suggest that clathrin contributes to stability of the ch-TOG protein early in the cell cycle. This effect on ch-TOG and the fact that clathrin inactivation can be phenocopied by inhibition of Aurora A kinase suggests that clathrin’s role in centrosome maturation involves its participation in a complex with TACC3 and ch-TOG, which has been shown to form upon Aurora A kinase phosphorylation of TACC3. This complex has so far been characterized at the mitotic spindle (Royle, 2012) as contributing to the stability of kinetochore microtubules. Here, we provide evidence of all three components at the interphase centrosome as well and propose that the low level of Aurora A activity before G2/M onset could initiate centrosomal formation of the complex. We further propose that the clathrin–TACC3–ch-TOG complex could contribute to the stability of centrosomal tubulin because we show that centrosomal loss of complex components induces γ-tubulin dispersion and centrosome fragmentation. Interestingly, TACC3 localization at the centrosome and TACC3 cell cycle levels were not affected by clathrin inactivation, suggesting that TACC3 is the recruiting component for the complex, as has been suggested for clathrin recruitment to the mitotic spindle (Royle, 2012). Inhibition of Aurora A kinase would also prevent TACC3 from recruiting clathrin and ch-TOG, explaining the shared phenotype with clathrin inactivation.
Although our data are consistent with clathrin’s centrosomal role involving its complexation with ch-TOG and TACC3, the possibility remains that clathrin also plays a membrane traffic function at the centrosome. Live-cell imaging shown here indicates that clathrin and pericentrin take the same route to the centrosome. The simplest interpretation of this data is that they both traffic along microtubules, a known pathway for delivering components to the maturing centrosome (Young et al., 2000). However, it is possible that clathrin-coated vesicles could serve as vehicles for delivering other centrosomal components in addition to clathrin itself. In light of this possibility, it is interesting that depletion of the clathrin adaptors ARH or epsin affects centrosome integrity (Lehtonen et al., 2008; Liu and Zheng, 2009). As adaptors link cargo to a clathrin-coated vesicle, their depletion is most likely to affect membrane traffic. In contrast, the other clathrin-associated protein, whose depletion causes centrosomal defects, is GAK, which plays a role in the clathrin-uncoating cycle (Shimizu et al., 2009). GAK loss could cause clathrin sequestration and thereby affect complexation with ch-TOG and TACC3.
Defects in all of the defined functions of CHC17 during the cell cycle could result in multinucleation. We observed that inactivation of CHC17 during S phase and at a later stage of mitosis resulted in considerably less multinucleation in one cell cycle than seen after 48–72 h of RNAi depletion of CHC17. Thus, the phenotype of multinucleation arises after cumulative loss of CHC17 functions during multiple rounds of mitosis. CHC22 depletion by RNAi resulted in an increase in multinucleated cells comparable with that induced by CHC17 depletion (Fig. 4). As CHC22 depletion did not affect centrosome number or maturation (Figs. 1 and 2) and endogenously expressed CHC22 does not associate with the mitotic spindle (Esk et al., 2010), it may influence later phases in mitosis that affect cytokinesis. A likely possibility is that CHC22’s distinct role in endosomal sorting (Esk et al., 2010) contributes to the extensive endosomal membrane traffic involved in midbody abscission (Montagnac et al., 2008; Prekeris and Gould, 2008) and that multinucleation arises from interfering with CHC22’s role in this traffic.
In summary, this study takes advantage of acute CHC17 clathrin inactivation within the cell cycle and complements prior studies involving lengthy RNAi treatment or deletion mutation. Considering all studies together suggests there are at least three temporally distinct processes by which clathrin function regulates mitotic progression. These are centrosome maturation (shown here), mitotic spindle stability (Royle et al., 2005; Royle and Lagnado, 2006; Lin et al., 2010), and cytokinesis (Niswonger and O’Halloran, 1997; Schweitzer et al., 2005). Here, we demonstrate that centrosome maturation requires CHC17 clathrin and that CHC22 clathrin influences the cell cycle independently, further establishing that functions of both clathrins are required for normal cell division.
Primary antibody concentrations ranged from 1–5 µg/ml for immunoblotting and IF assays. Antibodies specific for clathrin isoforms produced in our laboratory include mAb TD.1 against CHC17 (Näthke et al., 1992), mAb X22 against CHC17 (Brodsky, 1985a), rabbit pAb against CHC22 (Vassilopoulos et al., 2009), rabbit pAb against both CLCs (Acton et al., 1993), and mAb X16 against LCa (Brodsky, 1985a). A rabbit pAb against ch-TOG used for IF assays exclusively was a gift from L. Cassimeris (Lehigh University, Bethlehem, PA). Commercially available antibodies were used to detect β-actin (mAb; Sigma-Aldrich), γ-tubulin (mAb; Santa Cruz Biotechnology, Inc.), α-tubulin (mAb from Sigma-Aldrich and rabbit polyclonal from Santa Cruz Biotechnology, Inc.), PCNT2 (goat polyclonal; Santa Cruz Biotechnology, Inc.), centrin-2 (mAb; Abcam), SNAP-tag (rabbit polyclonals from both GenScript and New England Biolabs, Inc.), TACC3 (mAb; Santa Cruz Biotechnology, Inc.), ch-TOG (rabbit polyclonal from AbCam and rabbit polyclonal from BioLegend), PCM1 (rabbit polyclonal; Cell Signaling Technology), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; mAb; EMD Millipore), TfR (mAb; BD), and EEA1 (mAb; BD). Immunoblotting studies used secondary antibodies conjugated to HRP (Invitrogen) at 1:10,000 or 1:20,000. Secondary antibodies for IF included Alexa Fluor antibody conjugates 488, 555, and 647 (Molecular Probes or Invitrogen) and were used at 1:400 or 1:500.
The cDNA encoding nonneuronal human uLCa was cloned into plasmid DNA such that the SNAP-tag (New England Biolabs, Inc.) was incorporated at the N-terminal end of uLCa. The cDNA vector for GFP-pericentrin (pLPx7-GFP-pericentrin) was produced in the Doxsey laboratory at the University of Massachusetts Medical School.
HeLa cells stably expressing either GFP–α-tubulin or centrin-2–GFP were gifts from A. Straight (Stanford University, Stanford, CA) and M.-F.B. Tsou (Sloan-Kettering Institute, New York, NY), respectively. These cells were maintained in DME (Invitrogen) supplemented with 10% FBS, 100 U/ml penicillin, 100 µg/ml streptomycin, 10 mM Hepes, and 500 µg/ml G418 (Corning). Normal HeLa cells were maintained using the same media without G418.
Duplex siRNAs were synthesized (QIAGEN) based on experimentally validated target sequences for clathrin isoforms and subunits (Huang et al., 2004; Vassilopoulos et al., 2009), γ-tubulin (Hs_TUBG1_5, catalog no. S102780750), or nonsilencing siRNA (catalog no. 1027310). For RNAi treatments, cells were seeded (21,000 cells/cm2) in 6- or 24-well plates in Opti-MEM I reduced serum media (Invitrogen) and then transfected with duplex siRNAs (20 nM per treatment) complexed with HiPerFect (QIAGEN) in a 1:10 mixture (siRNA/HiPerFect). Conditions targeting CHC17 and CLCs together used a combination of 10 nM CHC17 and 10 nM CLC siRNA. CLC siRNA treatments included two parts siRNA-targeting LCa combined with one part siRNA-targeting LCb. CHC22 depletion was achieved by combining two siRNAs (1:1), as previously described (Vassilopoulos et al., 2009). 6 h after siRNA transfection, cells were returned to normal growth conditions and then harvested for analysis 48 to 72 h later. Targeting and nontargeting siRNA effects were confirmed by immunoblotting.
HeLa cells were seeded (50,000 cells/cm2) in 6-well plates 1 d before plasmid transfection (5 h at 37°C in 5% CO2) using Lipofectamine 2000 (2.0 µg plasmid in 5 µl Lipofectamine 2000) and Opti-MEM I reduced serum media according to the manufacturer’s instructions (Invitrogen). For 48 h after transfection, cells were cultured under nonselective conditions, and then they were passaged in DME supplemented with 10% FBS, 10 mM Hepes, 100 U/ml penicillin, 100 µg/ml streptomycin, and 700 µg/ml G418 (G418 selection media). Single colonies were selected 1.5–2 wk after adding G418 selection media. Sterile glass cylinders (Thermo Fisher Scientific) were stamped in autoclaved vacuum grease (Corning) to trypsinize single resistant colonies for expansion. Clonal cell expression SNAP-uLCa was validated by immunoblotting, IF, and IP.
IF protocols were modified from established methods (Piehl and Cassimeris, 2003; Straight et al., 2005). Cells grown on a 0.17-mm coverglass (Corning) were fixed with 4% PFA (Electron Microscopy Sciences) diluted in cytoskeleton stabilization buffer (10 mM MES, pH 6.1, 138 mM KCl, 3 mM MgCl, and 2 mM EGTA) for 20 min at room temperature. Fixation was quenched with 25 mM glycine in PBS. To detect TACC3, ch-TOG, and clathrin at centrosomes, cells were fixed in −20°C methanol with 1 mM EDTA (10 min at room temperature; Piehl and Cassimeris, 2003). All fixed cells were permeabilized (10 min at room temperature) with TBS containing 0.5% Triton X-100, pH 7.4, washed two times with TBS–0.1% Triton X-100, pH 7.4, and blocked in antibody dilution buffer (Abdil; TBS containing 0.1% Triton X-100 and 2% wt/vol BSA, pH 7.4, for 10 min at room temperature). Primary antibodies (1–5 µg/ml in Abdil) were applied (2 h at room temperature), and, after washing with TBS containing 0.1% Triton X-100, Alexa Fluor secondary antibodies suspended in Abdil were applied (45 min at room temperature). Cells were then washed in TBS containing 0.1% Triton X-100 followed by TBS alone. Where indicated, cells were incubated with 300 nM DAPI to stain DNA (10 min at room temperature) and then washed in TBS. Cells were mounted for microscopy in 2.5% DABCO (Sigma-Aldrich), 50 mM TBS, pH 8.0, and 90% glycerol.
Cells processed by IF were analyzed by confocal laser-scanning microscopy using an inverted system (DM1 6000 CS, SP5; Leica) with oil immersion objectives (63× 1.4 NA and 100× 1.4 NA; both HCX Plan Apochromat; Leica) and argon (488) and HeNe (543 and 633) lasers. Images were acquired using LAS AF SP5 software (Leica) in sequential scan mode with a 600-Hz scan rate, line averages of four to six, and a 512 × 512– or 1,024 × 1,024–pixel resolution. Raw images were processed using LAS AF SP5, ImageJ (version 1.45c; National Institutes of Health), and Photoshop (CS3; Adobe). To improve image quality, raw images were processed using a median filter (LAS AF SP5) before any analysis was performed. Colocalization measurements were performed using LAS AF SP5, and fluorescence distribution measurements were performed on maximum projections of 3D images using the Radial Profile Extended plug-in (version 1, by Philippe Carl) in ImageJ (version 1.45c). Adjustments to brightness and contrast of images were restricted to the dynamic range of the fluorescent intensity profile for merged fluorophores, applied to the whole image, and the same settings were maintained for all samples within an experiment.
Asynchronous cells were seeded (50,000 cells/cm2) in 35-mm culture dishes 1 d before transfection with the GFP-pericentrin–encoding plasmid pLPx7-GFP-pericentrin using Lipofectamine 2000 (0.8-µg plasmid in 2 µl Lipofectamine 2000) and Opti-MEM I reduced serum media according to manufacturer’s instructions. After transfection, the Opti-MEM I transfection media was removed, and cells were cultured in DME media supplemented with 10% FBS, 100 U/ml penicillin, 100 µg/ml streptomycin, and 10 mM Hepes for 48 h. Cells were then labeled with SNAP-TMR-Star (3 µM in phenol red–free DME with 10% FBS) for 30 min at 37°C according to manufacturer’s instructions (New England Biolabs, Inc.). Cells were then detached from culture dishes using PBS-based Cell Dissociation Buffer (Invitrogen), pelleted (1,000 g for 5 min), resuspended in phenol red–free DME with 10% FBS, and seeded in Lab-Tek II chambered coverglass (0.17-mm glass thickness; Thermo Fisher Scientific). This transfer step avoided residual SNAP-TMR-Star sticking to the coverglass surface. Cells were incubated (37°C in 5% CO2 with 95% humidity) for 5 h before initiating time-lapse imaging.
SNAP-TMR-Star–labeled cells transiently expressing GFP-pericentrin were analyzed by confocal laser-scanning microscopy using an inverted system (DM1 6000 CS, SP5) with photomultiplier tube detection, an incubation chamber (37°C in 5% CO2), an oil immersion objective (63× 1.4 NA; HCX Plan Apochromat), and argon (488 laser power, 2%) and HeNe (543 laser power, 10%) lasers. Images were acquired using LAS AF SP5 in xyt-sequential scan mode (10-s or 10-min scanning intervals), a 700-Hz scan rate, line averages of two, and a 512 × 512–pixel resolution. Raw images were processed using a median filter in ImageJ (version 1.45c). Adjustments to brightness and contrast of images were performed in Photoshop (CS3), restricted to the dynamic range of the fluorescent intensity profile for merged fluorophores, and applied to the whole xyt image sequence series, and the same settings were maintained for all samples within an experiment.
Cell lysates were resolved by SDS-PAGE (NuPage Bis-Tris 4–12% SDS-acrylamide gels; Invitrogen), transferred to nitrocellulose membranes (EMD Millipore), and exposed to primary (1–5 µg/ml) and peroxidase-conjugated secondary antibodies. Labeled proteins were detected using the ECL-Plus reagent (GE Healthcare). Molecular mass markers (Rainbow; GE Healthcare) were used for calibration, and protein band intensities were quantified using Quantity One (Bio-Rad Laboratories) or ImageJ (version 1.45c) software. For detection of the SNAP-tag and CHC17 by blotting, the anti–SNAP-tag antibody (New England Biolabs, Inc.) and TD.1 antibody were used, respectively.
The X22 mAb was used to immunoprecipitate CHC17 clathrin. IgG mAb (IgG1, κ; BD) was used as a control. IP was performed from cell lysate prepared in high-Tris IP lysis buffer (0.5 M Tris, pH 7.2, 1.0% Triton X-100, 20 mM EDTA, 5 µg/ml aprotinin, 10 µg/ml leupeptin, 10 µg/ml pepstatin A, and 1 mM PMSF), conditions that disassemble clathrin coats (Wilde and Brodsky, 1996). Lysates were diluted in IP lysis buffer to a standard concentration of 2 mg/ml and 100 µl used for each specific IP, first preclearing with protein G Sepharose (GE Healthcare), then incubating with 2 µg of primary antibody overnight at 4°C and then with protein G for 1 h at 4°C followed by washing (3×) with 500 µl of IP lysis buffer. Precleared and IP samples were resolved by SDS-PAGE, transferred to nitrocellulose membranes, and analyzed by immunoblotting.
For in vivo labeling of SNAP-tagged uLCa fusion proteins, live cells plated on coverslips were incubated (30 min at 37°C in 5% CO2) with 3 µM SNAP-TMR-Star (New England Biolabs, Inc.) diluted in normal growth media with serum. Cells were then washed three times with normal growth media with serum, incubated (37°C in 5% CO2) an additional 30 min, and then imaged using confocal laser-scanning microscopy, as detailed under Time-lapse confocal microscopy imaging.
Covalent cross-linking of SNAP-uLCa was achieved using BG-GLA-BG, a SNAP-tag homodimerizer shown in Fig. S5, synthesized at New England Biolabs, Inc. following established methods (Lemercier et al., 2007). Stable HeLa-SNAP-uLCa clones were treated at specified concentrations (5–10 µM) with BG-GLA-BG dissolved in DMSO and then diluted in normal growth media with serum and incubated (2 h at 37°C in 5% CO2).
For treatment with the Aurora A kinase inhibitor MLN8054 (Selleck Chemicals) and comparison with BG-GLA-BG treatment, HeLa-SNAP-uLCa clone 3.3 cells were synchronized to S phase by T/T and then treated at 0 h (T0) after T/T with DMSO (mock) for 4 or 8 h, 10 µM BG-GLA-BG for 2 h, or 500 nM MLN8054 for 4 or 8 h. At 4 and 8 h, cells were fixed with methanol containing 1 mM EDTA (10 min) and processed for IF.
Cells that were incubated in the presence or absence of homodimerizer were placed on ice, washed three times with ice-cold HBSS (Invitrogen), and then exposed to 50 µg/ml Alexa Fluor 488–conjugated transferrin (Invitrogen) in HBSS (30–60 min at 4°C). After washing (four times with HBSS at 4°C), prewarmed HBSS was added to cells, and cells were incubated (37°C in 5% CO2) for 10 min to 1 h to allow for transferrin internalization. Immediately after this incubation, the cells were fixed at room temperature with 4% PFA and processed for IF.
Cell surface biotin labeling and receptor internalization assays were modified from established methods (Blagoveshchenskaya et al., 2002). HeLa-SNAP-uLCa clone 3.3 cells were seeded in five 60-mm dishes (80,000 cells/cm2) 1 d before treating them with 5 µM BG-GLA-BG or DMSO (mock) for 2 h at 37°C, 5% CO2, and 95% humidity. Cells were washed on ice with ice-cold PBS, pH 8.0, and then treated with 10 mM sulfo-NHS-SS-biotin (Thermo Fisher Scientific) for 30 min at 4°C. Unreacted biotin was quenched by washing cells with ice-cold PBS, pH 7.2, containing 50 µM glycine. Two dishes representing 0 min (0*, one to not be stripped of biotin, and 0, to be stripped of biotin) were kept on ice while the other remaining three dishes were treated with prewarmed PBS containing Ca2+ and Mg2+ and placed in a cell incubator for 5–15 min at 37°C, and then the reaction was stopped with 5 mg/ml iodoacetamide on ice. Cell lysates were prepared in high-Tris IP lysis buffer after washing with ice-cold PBS and exposed to SA agarose resin (Thermo Fisher Scientific) for 1 h at 4°C to pull down internalized biotin-labeled receptors. SA-bound samples representing plasma membrane–associated (0 min) and internalized (5, 10, and 15 min) receptors were pelleted by centrifugation and then washed five times with 1% NP-40 in PBS. SA-bound samples and total cell lysate for all time points were immunoblotted for TfR and GAPDH. To quantify the extent of internalized TfR, blotting signals were assessed by densitometry, and values defined for all time points were expressed in arbitrary units using the following equation:
Cells were synchronized to the G1/S-phase boundary using the T/T method (Bello, 1969). HeLa clones were seeded (15,000 cells/cm2) in 10-cm dishes 1 d before treatment with 2 mM thymidine in normal G418 selection media for 19 h. Cells were released from this first thymidine block by washing with PBS (two times) followed by incubation in G418 selection media without thymidine for 9 h. A second thymidine block was then performed for an additional 16 h before returning cells to G418 selection media without thymidine. Cell synchronization was confirmed by flow cytometry after labeling cells with propidium iodide (Fig. S4). Synchronized cells were used in cross-linking experiments at varying time intervals after the second thymidine release was initiated (2, 4, 6, 8, and 10 h).
Synchronized cells were harvested by trypsinization (0.05% trypsin-EDTA; Invitrogen), centrifuged, and washed two times with ice-cold PBS. Cell pellets were fixed by slowly adding 70% ethanol (chilled at −20°C) while vortexing and then stored at 4°C. On the day of flow cytometry analysis, fixed cells were pelleted and washed two times with ice-cold PBS to remove ethanol. The supernatant was removed, and pellets were left to dry to avoid ethanol contamination. Cell pellets were then resuspended in PBS containing 40 µg/ml propidium iodide and 40 µg/ml ribonuclease A and incubated in the dark for 30–60 min at 37°C. Cells were analyzed for DNA content using a flow cytometer (BD). Data were processed using FlowJo software (version 9.4.11; Tree Star) to determine the proportion of cells in each phase of the cell cycle (Kawamoto et al., 1979).
Data were statistically analyzed using GraphPad Prism software (GraphPad Software). For nonparametric data, statistical analyses were performed using a one-way analysis of variance Friedman’s matched pairs test and the Dunn’s multiple comparison post-hoc test (90% confidence interval). A regular one-way analysis of variance was performed for data that passed the normality test, D’Agostino–Pearson omnibus test, and the post-hoc test was Tukey’s multiple comparison test (95% confidence interval).
Fig. S1 shows quantification of the knockdown effects of all siRNA sequences used in this study, demonstrating reproducible effects on different transfected HeLa cell clones when compared with nonsilencing siRNA. Fig. S2 shows binding of the SNAP-uLCa fusion protein to CHC17 in HeLa-SNAP-uLCa clones and colocalization with endogenous CHC17 clathrin in cells representing all phases of the cell cycle. Figs. S3 and S4 show that acute inactivation of clathrin by SNAP-tag–mediated cross-linking in clone 3.3 cells did not significantly affect CHC17, CHC22, or CLC levels, appearance of multinuclear cells within one round of mitosis, or cell cycle progression but did induce a multipolar spindle phenotype within one cell cycle. Fig. S5 shows the chemical structure of BG-GLA-BG. Video 1 shows a live-cell image of clathrin colocalization with GFP-pericentrin at an interphase centrosome and in local vesicular structures at 10-min intervals over 2 h. Video 2 shows a live-cell image of colocalized clathrin and GFP-pericentrin in small structures moving toward the centrosome of a G2/M-phase–transitioning cell at 10-s intervals over 2 min. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.201205116/DC1.
|Abbreviations used in this paper:|
|CHC||clathrin heavy chain|
|ch-TOG||colonic, hepatic tumor overexpressed gene|
|CLC||clathrin light chain|
|GAK||cyclin G–associated kinase|
|GAPDH||glyceraldehyde 3-phosphate dehydrogenase|
We thank Rebecca Heald (University of California, Berkeley, Berkeley, CA) for support and advice, Aaron Straight for helpful discussion and for the stable HeLa-GFP–α-tubulin cell line, Meng-Fu Bryan Tsou for the stable HeLa–centrin-2–GFP cell line, and Lynne Cassimeris for her advice and the ch-TOG antibody. We also thank Brenda Baker (New England Biolabs, Inc.) for assistance with synthesis of BG-GLA-BG.
This work was supported by National Institutes of Health grants GM038093 to F.M. Brodsky and GM051994 to S.J. Doxsey, National Institutes of Health training grant NCI T32 CA09043 to A.B. Foraker, and an Arthritis Foundation postdoctoral fellowship to T.M. Evans.
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