Document Detail


Clathrin pit-mediated endocytosis of neutrophil elastase and cathepsin G by cancer cells.
MedLine Citation:
PMID:  22915586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neutrophil elastase (NE) is a neutrophil-derived serine proteinase with broad substrate specificity. We have recently demonstrated that NE is capable of entering tumor cell endosomes and processing novel intracellular substrates. In the current study, we sought to determine the mechanism by which NE enters tumor cells. Our results show that NE enters into early endosomal antigen-1(+) endosomes in a dynamin- and clathrin-dependent but flotillin-1- and caveolin-1-independent fashion. Cathepsin G (but not proteinase-3) also enters tumor endosomes via the same mechanism. We utilized (125)I-labeled NE to demonstrate that NE binds to the surface of cancer cells. Incubation of radiolabeled NE with lung cancer cells displays a dissociation constant (K(d)) of 284 nm. Because NE is known to bind to heparan sulfate- and chondroitin sulfate-containing proteoglycans, we treated cells with glycanases to remove these confounding factors, which did not significantly diminish cell surface binding or endosomal entry. Thus, NE and CG bind to the surface of cancer cells, presumably to a cell surface receptor, and subsequently undergo clathrin pit-mediated endocytosis.
Authors:
Alyssa D Gregory; Pamela Hale; David H Perlmutter; A McGarry Houghton
Related Documents :
3606776 - Prediction of cervical neoplasia diagnosis groups. discriminant analysis on digitized c...
15324446 - Benign glandular cells in posthysterectomy vaginal smears: the incidence is higher than...
19821496 - Significance of cell proliferation markers (minichromosome maintenance protein 7, topoi...
2321456 - Conjunctival brush cytology.
10905676 - Wound cell variation in closed surgical wounds as measured with two independent methods.
17911156 - Fruit flies and the sperm proteome.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-22
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2012-12-31     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35341-50     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, 15261, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cathepsin G / genetics,  metabolism*
Caveolin 1 / genetics,  metabolism
Chondroitin Sulfates / genetics,  metabolism
Clathrin / genetics,  metabolism*
Coated Pits, Cell-Membrane / genetics,  metabolism*
Cricetinae
Cricetulus
Endocytosis*
Humans
Leukocyte Elastase / genetics,  metabolism*
Lung Neoplasms / genetics,  metabolism*
Neoplasm Proteins / genetics,  metabolism*
Protein Binding / genetics
Protein Transport / genetics
Grant Support
ID/Acronym/Agency:
5T32HL007563-24/HL/NHLBI NIH HHS; R01 HL108979/HL/NHLBI NIH HHS; R01HL108979/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Caveolin 1; 0/Clathrin; 0/Neoplasm Proteins; 9007-28-7/Chondroitin Sulfates; EC 3.4.21.20/CTSG protein, human; EC 3.4.21.20/Cathepsin G; EC 3.4.21.37/Leukocyte Elastase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Soluble prion protein inhibits amyloid-? (A?) fibrillization and toxicity.
Next Document:  Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/ph...