Document Detail


Clathrin-mediated endocytosis: the physiological mechanism of vesicle retrieval at hippocampal synapses.
MedLine Citation:
PMID:  17599959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The maintenance of synaptic transmission requires that vesicles are recycled after releasing neurotransmitter. Several modes of retrieval have been proposed to operate at small synaptic terminals of central neurons, but the relative importance of these has been controversial. It is established that synaptic vesicles can collapse on fusion and the machinery for retrieving this membrane by clathrin-mediated endocytosis (CME) is enriched in the presynaptic terminal. But it has also been suggested that the majority of vesicles released by physiological stimulation are recycled by a second, faster mechanism called 'kiss-and-run', which operates in 1 s or less to retrieve a vesicle before it has collapsed. The most recent evidence argues against the occurrence of 'kiss-and-run' in hippocampal synapses. First, an improved fluorescent reporter of exocytosis (sypHy), indicates that only a slow mode of endocytosis (tau = 15 s) operates when vesicle fusion is triggered by a single nerve impulse or short burst. Second, this retrieval mechanism is blocked by overexpressing the C-terminal fragment of AP180 or by knockdown of clathrin using RNAi. Third, vesicle fusion is associated with the movement of clathrin and vesicle proteins out of the synapse into the neighbouring axon. These observations indicate that clathrin-mediated endocytosis is the major, if not exclusive, mechanism of retrieval in small hippocampal synapses.
Authors:
Björn Granseth; Benjamin Odermatt; Stephen J Royle; Leon Lagnado
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-06-28
Journal Detail:
Title:  The Journal of physiology     Volume:  585     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-02-25     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  681-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Clathrin / physiology*
Endocytosis / physiology*
Hippocampus / physiology*
Humans
Nerve Tissue Proteins / physiology
Signal Transduction / physiology
Synapses / physiology*
Synaptic Transmission / physiology
Synaptophysin / physiology
Grant Support
ID/Acronym/Agency:
MC_U105178794//Medical Research Council
Chemical
Reg. No./Substance:
0/Clathrin; 0/Nerve Tissue Proteins; 0/Synaptophysin
Comments/Corrections

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