| Clathrin-dependent trafficking of subtilase cytotoxin, a novel AB5 toxin that targets the endoplasmic reticulum chaperone BiP. | |
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MedLine Citation:
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PMID: 18042253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Subtilase cytotoxin (SubAB) is the prototype of a new family of AB5 cytotoxins produced by Shiga toxigenic Escherichia coli. Its cytotoxic activity is due to its capacity to enter cells and specifically cleave the endoplasmic reticulum (ER) chaperone BiP. However, its trafficking within target cells has not been investigated previously. In Vero cells, fluorescence colocalization with subcellular markers established that SubAB is trafficked from the cell surface to the ER via a retrograde pathway similar, but not identical, to those of Shiga toxin (Stx) and cholera toxin (Ctx), with their pathways converging at the Golgi. The clathrin inhibitor phenylarsine oxide prevented SubAB entry and BiP cleavage in SubAB-treated Vero, HeLa and N2A cells, while cholesterol depletion did not, demonstrating that, unlike either Stx or Ctx, SubAB internalization is exclusively clathrin-dependent. |
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Authors:
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Damien C Chong; James C Paton; Cheleste M Thorpe; Adrienne W Paton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-11-27 |
Journal Detail:
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Title: Cellular microbiology Volume: 10 ISSN: 1462-5822 ISO Abbreviation: Cell. Microbiol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-08 Completed Date: 2008-03-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 795-806 Citation Subset: IM |
Affiliation:
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School of Molecular and Biomedical Science, University of Adelaide, SA 5005, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arsenicals / pharmacology Cercopithecus aethiops Clathrin / antagonists & inhibitors, metabolism* Endoplasmic Reticulum / chemistry, enzymology* Enzyme Inhibitors / pharmacology Escherichia coli Proteins / metabolism* Golgi Apparatus / chemistry Heat-Shock Proteins / antagonists & inhibitors* Hela Cells Humans Microscopy, Fluorescence Molecular Chaperones / antagonists & inhibitors* Shiga-Toxigenic Escherichia coli / physiology* Subtilisins / metabolism* Vero Cells |
| Grant Support | |
ID/Acronym/Agency:
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P30DK-34928/DK/NIDDK NIH HHS; R01AI-068715/AI/NIAID NIH HHS; R01AI-59509/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arsenicals; 0/Clathrin; 0/Enzyme Inhibitors; 0/Escherichia coli Proteins; 0/Heat-Shock Proteins; 0/Molecular Chaperones; 0/molecular chaperone GRP78; 637-03-6/oxophenylarsine; EC 3.4.21.-/Subtilisins; EC 3.4.21.-/subtilase cytotoxin, E coli |
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