| Clastogenicity of isoamylene oxide to rat lymphocytes in culture. | |
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MedLine Citation:
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PMID: 7596369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mutagenic activity of the aliphatic epoxide isoamylene oxide (2-methyl-2,3-epoxybutane) is not readily detectable in the standard Ames test. In this study, the clastogenic potential of isoamylene oxide was evaluated using an in vitro mammalian cell culture system. Approximately 48 h after establishing primary cultures of rat lymphocyte cultures, the cells were treated for 4 h with various concentrations of isoamylene oxide (50, 166.7, 500,, 1666.7, and 5000 micrograms/ml in the initial assay and 500, 1000, 2000, 3000, 4000, and 5000 micrograms/ml in the confirmatory assay). The cultures were harvested 24 h after termination of the treatment. Based upon the mitotic indices, cultures treated with the three highest concentrations in both the initial and confirmatory assays were evaluated to estimate the chromosomal aberration frequencies. Isoamylene oxide demonstrated a strong clastogenic activity in this assay: up to 29% aberrant cells (without gaps) were observed at the highest concentration analyzed. The presence of an external metabolic activation system (S9) did not seem to influence the magnitude of the response at the dose levels analyzed. |
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Authors:
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B B Gollapudi; V A Linscombe; J W Wilmer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 347 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-08-03 Completed Date: 1995-08-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 9-12 Citation Subset: IM |
Affiliation:
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Dow Chemical Company, Midland, MI 48674, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Chi-Square Distribution Chromosome Aberrations Dose-Response Relationship, Drug Epoxy Compounds / toxicity* Lymphocytes / drug effects Male Mitotic Index Mutagenicity Tests Mutagens / toxicity* Rats Rats, Sprague-Dawley Statistics as Topic |
| Chemical | |
Reg. No./Substance:
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0/Epoxy Compounds; 0/Mutagens; 5076-19-7/beta-isoamylene oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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