Document Detail


Clastogenic effects of methylnitrosourea and ethylnitrosourea on chromosomes from human fibroblast cell lines.
MedLine Citation:
PMID:  1264107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human fibroblast cell lines were pulse-treated for 1 h with either methylnitrosourea (MNU) or ethylnitrosourea (ENU) at various time intervals before harvesting for chromosome analysis. Cells treated with 1 X 10(-3) M, 5 X 10(-4) M, and 1 X 10(-4) M final concentrations of MNU and ENU during the G2 or M phases of the cell cycle showed a significant increase in chromatid-type abnormalities over controls. Cells exposed to MNU or ENU 23 h before harvest showed some chromosome-type abnormalities, reflecting probable damage induced during the G1 phase of the cell cycle or derived from chromatid damage induced during the previous cell cycle. The mitotic indices and incidences of abnormalities suggested a dose response effect when cells were treated with the two higher concentrations and the three concentrations, respectively, of MNU or ENU. Chromatid abnormalities were observed in MUN and ENU-treated cells from each of four cell lines. From this investigation, it was concluded that MNU and ENU treatment of human diploid cell lines in vitro induced both chromatid and chromosome aberrations. MNU and ENU, both of which had previously been shown to be mutagenic in experimental animals, are, therefore, also considered to be mutagenic at the chromosome level in human fibroblasts grown and treated in cell culture.
Authors:
W G Sanger; J D Eisen
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  34     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-07-06     Completed Date:  1976-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  415-26     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Chromosome Aberrations*
Chromosomes / drug effects*
Dose-Response Relationship, Drug
Ethylnitrosourea / toxicity*
Methylnitrosourea / toxicity*
Mitotic Index / drug effects
Nitrosourea Compounds / toxicity*
Chemical
Reg. No./Substance:
0/Nitrosourea Compounds; 684-93-5/Methylnitrosourea; 759-73-9/Ethylnitrosourea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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