| Classification of test agent-specific effects in the Syrian hamster embryo assay (pH 6.7) using infrared spectroscopy with computational analysis. | |
| | |
MedLine Citation:
|
PMID: 22362182 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The Syrian hamster embryo (SHE) cell transformation assay (pH 6.7) has utility in the assessment of potential chemical carcinogenicity (both genotoxic and non-genotoxic mechanisms of action). The assay uses morphological transformation as an end point and has a reported sensitivity of 87%, specificity of 83% and overall concordance of 85% with in vivo rodent bioassay data. However, the scoring of morphologically transformed SHE cells is subjective. We treated SHE cells grown on low-E reflective slides with benzo[a]pyrene, 3-methylcholanthrene, anthracene, N-nitroso-N-methylnitroguanidine, ortho-toluidine HCl, 2,4-diaminotoluene or D-mannitol for 7 days before fixation with methanol. Identified colonies were interrogated by acquiring a minimum of five infrared (IR) spectra per colony using attenuated total reflection Fourier-transform IR spectroscopy. Individual IR spectra were acquired over a spatial area of approximately 250 × 250 μm. Resultant data were analysed using Fisher's linear discriminant analysis and feature histogram algorithms to extract classifying biomarkers of test agent-specific effects or transformation in SHE cells. Clustering of spectral points suggested co-segregation or discrimination of test agent categories based on mechanism of action. Towards transformation, unifying alterations were associated with alterations in the Amide I and Amide II peaks; these were consistently major classifying biomarkers for transformed versus non-transformed SHE cells. Our approach highlights a novel method towards objectively screening and classifying SHE cells, be it to ascertain test agent treatment based on mechanism of action or transformation. |
| | |
Authors:
|
Abdullah A Ahmadzai; Júlio Trevisan; Weiyi Pang; Imran I Patel; Nigel J Fullwood; Shannon W Bruce; Kamala Pant; Paul L Carmichael; Andrew D Scott; Francis L Martin |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-28 |
Journal Detail:
|
Title: Mutagenesis Volume: - ISSN: 1464-3804 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8707812 Medline TA: Mutagenesis Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Centre for Biophotonics, Lancaster Environment Centre. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical, Laboratory, and Pacing Predictors of CRT Response.
Next Document: The mode of binding ACMA-DNA relies on the base-pair nature.