Document Detail

Classification of normal colorectal mucosa and adenocarcinoma by morphometry.
MedLine Citation:
PMID:  3666675     Owner:  NLM     Status:  MEDLINE    
Semi-automatic image analysis was used to make a morphometrical assessment of 15 nuclear and cellular variables in normal (n = 20) and malignant (n = 30) colorectal epithelium. Principal components analysis on the matrix of correlations between variables identified four main sources of variation within the dataset. These were, in decreasing order of importance: (1) nuclear size, nuclear cytoplasmic ratio and nuclear position within the cell; (2) the variability of nuclear size; (3) nuclear elongation and polarity; (4) nuclear shape and its variation. Discriminant analysis was conducted between histologically normal mucosa (n = 10) and adenocarcinoma in ulcerative colitis (n = 20). Using stepwise variable selection, the mean nuclear cytoplasmic ratio (normal, mean 20.4 (s.d. +/- 2.0); tumour, mean 39.7 (s.d. +/- 7.0)) and the coefficient of variation of nucleus to cell apex distance (normal, mean 19.2 (s.d. +/- 7.5); tumour, mean 47.8 (s.d. +/- 9.1)) were chosen as discriminating features. They were used to derive a discriminant function which gave perfect discrimination between the two groups. Scatter plots of these two variables confirmed complete separation of normal mucosa from adenocarcinoma and provided a simple method of applying the discriminant function. Discriminatory performance did not deteriorate when the function was applied to further normals (n = 10) and adenocarcinoma (n = 10). This study highlights the descriptive differences between normal and malignant colorectal epithelium and shows that case allocation may be made to these two lesion categories using a morphometrically-derived classification rule.
P W Hamilton; D C Allen; P C Watt; C C Patterson; J D Biggart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histopathology     Volume:  11     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1987-12-17     Completed Date:  1987-12-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  901-11     Citation Subset:  IM    
Department of Pathology, Royal Victoria Hospital, Queen's University of Belfast, Northern Ireland.
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MeSH Terms
Adenocarcinoma / classification*,  ultrastructure
Cell Nucleus / ultrastructure
Colon / ultrastructure*
Colonic Neoplasms / classification*,  ultrastructure
Cytoplasm / ultrastructure
Image Processing, Computer-Assisted
Intestinal Mucosa / ultrastructure*
Rectal Neoplasms / classification*,  ultrastructure
Rectum / ultrastructure*
Statistics as Topic

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