Document Detail


Classification of M nontypeable group A streptococcus with the use of field inversion gel electrophoresis.
MedLine Citation:
PMID:  14692226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Group A streptococcus (GAS) are traditionally classified based on M and T protein antigens. However, many strains do not react with available M antisera and are classified as M nontypeable (M NT). M and T typing, field inversion gel electrophoresis (FIGE), and 5' emm gene sequencing were performed on 24 M NT GAS isolates. FIGE patterns of the M NT isolates were compared by visual inspection and by computer analysis with the patterns of 139 isolates representing 72 M-types of GAS. Seven different FIGE patterns (I-VII) were seen among the M NT isolates. FIGE patterns I and III were identical or closely related to patterns seen with M12 and M22 isolates. The computer analysis determined the following relationships: pattern IV to M5, pattern VI to M61, and pattern VII to M59. Thus, the emm gene sequence correlated with the computer analysis of the FIGE pattern for each isolate. FIGE can be useful to help distinguish clinical isolates of GAS in an epidemiological study.
Authors:
Judith M Martin; Karen A Barbadora; Ellen R Wald; Michael Green
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Pediatric pathology & molecular medicine     Volume:  22     ISSN:  1522-7952     ISO Abbreviation:  Pediatr Pathol Mol Med     Publication Date:    2003 Jul-Aug
Date Detail:
Created Date:  2003-12-24     Completed Date:  2004-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100885435     Medline TA:  Pediatr Pathol Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  303-9     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Pittsburgh School of Medicine, Division of Allergy, Immunology, and Infectious Diseases, Children's Hospital of Pittsburgh, 3705 Fifth Ave. at DeSoto Street, Pittsburgh, PA 15213, USA. judy.martin@chp.edu
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MeSH Terms
Descriptor/Qualifier:
DNA, Bacterial / analysis*,  genetics
Electrophoresis, Gel, Pulsed-Field*
Genes, Bacterial*
Image Processing, Computer-Assisted
Reference Standards
Sequence Analysis, DNA
Serotyping
Streptococcus pyogenes / classification*,  genetics*,  isolation & purification
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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