| Classical swine fever virus suppresses maturation and modulates functions of monocyte-derived dendritic cells without activating nuclear factor kappa B. | |
| | |
MedLine Citation:
|
PMID: 21764089 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Classical swine fever virus (CSFV) compromises the host immune system, causing the severe disease of pigs. Dendritic cells (DCs) are the most potent inducers of immune responses. In the present study, we investigated the functional properties of porcine monocyte-derived DCs (Mo-DCs) affected by CSFV. Results showed that the expression of surface markers of DCs such as major histocompatibility complex class II (MHC-II), CD80, CD83 and CD86 were unimpaired, but an obviously increased expression of CD172a in DCs was noticed 48h after CSFV infection. The expression profiles of cytokines were detected in cultured Mo-DCs after various treatments for 48h by Q-RT-PCR. The findings suggested that CSFV infection significantly increased the mRNA expression of IL-10 and TNF-α, and inhibited IL-12 expression, with little effect on IFN-α and IFN-γ expression. We further demonstrated that CSFV was incapable of activating the nuclear factor kappa B (NF-κB) in infected DCs, which was characterized by an unvaried DNA binding activity of NF-κB, the lack of translocation of p65/RelA from the cytoplasm to the nucleus and the stabilization of p65/RelA expression. Furthermore, Western blot analysis indicated that the inactivation of NF-κB was due to the failure of IκBα degradation. The data demonstrated that CSFV could be replicated in DCs and CSFV infection could modulate the secretion of crucial co-stimulatory molecules and cytokines which down-regulated maturation of DCs, without activating NF-κB in DCs. Thus, the results suggested a possible mechanism for CSFV evasion of innate host defenses, providing the basis for understanding molecular pathways in CSFV pathogenesis. |
| | |
Authors:
|
Li-Jun Chen; Xiao-Ying Dong; Hai-Yan Shen; Ming-Qiu Zhao; Chun-Mei Ju; Lin Yi; Xue-Tao Zhang; Yan-Mei Kang; Jin-Ding Chen |
Related Documents
:
|
21630249 - Antigen presentation capacity of dendritic cells is impaired in ongoing enterovirus-myo... 10744679 - Regulation of fasl by nf-kappab and ap-1 in fas-dependent thymineless death of human co... 18291569 - Protective effect of saponins derived from the roots of platycodon grandiflorum against... 12270729 - Absence of weight loss during cryptosporidium infection in susceptible mice deficient i... 15599929 - Fas/fasl interaction: a novel immune therapy approach with immobilized biologicals. 9566799 - Up-regulation of fas ligand (fasl) mrna expression in peripheral blood mononuclear cell... 6460939 - In vitro cytochemical and cytophysiological study of in vivo activated mouse peritoneal... 16424939 - Peptide vaccination of mice immune to lcmv or vaccinia virus causes serious cd8 t cell-... 2191489 - Immunohistochemical localization of pasteurella haemolytica a1-derived endotoxin, leuko... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-15 |
Journal Detail:
|
Title: Research in veterinary science Volume: - ISSN: 1532-2661 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401300 Medline TA: Res Vet Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Crown Copyright © 2011. Published by Elsevier India Pvt Ltd. All rights reserved. |
Affiliation:
|
College of Veterinary Medicine, South China Agricultural University, 483 Wu Shan Road, Tian He District, Guangzhou 510642, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Can informed choice invitations lead to inequities in intentions to make lifestyle changes among par...
Next Document: Lactobacillus reuteri ATCC 55730 and L22 display probiotic potential in vitro and protect against Sa...