| Clara cell secretory protein increases phagocytic and decreases oxidative activity of neutrophils. | |
| | |
MedLine Citation:
|
PMID: 20728946 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Horses suffer from recurrent airway obstruction, an asthma-like condition induced by repeat inhalation of environmental substances present in barn air. Clara cell secretory protein (CCSP) is much reduced during active inflammation when neutrophils predominate in the airways, and in chronic asthmatics. We sought to investigate morphologic and functional interactions of CCSP with neutrophils. Bronchoalveolar and blood neutrophils from healthy control animals, and from animals with recurrent airway obstruction in remission and exacerbation, were evaluated by immuno-cytochemistry and immuno-electron microscopy for presence of CCSP. Blood neutrophil oxidative burst and phagocytic activities were determined in the presence of different concentrations of recombinant equine CCSP. Bronchoalveolar lavage neutrophils from horses with exacerbated lung inflammation, but not from control horses, and not blood neutrophils from either group of animal, contained abundant immunoreactive CCSP. On immuno-electron microscopy, CCSP localized to the cytoplasm and nucleus. Incubation of blood neutrophils with CCSP significantly reduced oxidative burst activity (P<0.0001) and increased phagocytosis (P<0.001) of neutrophils. These findings indicate that CCSP enters neutrophils in horses with active neutrophilic lung inflammation and alters the function of neutrophils in blood. Presence in the nucleus suggests a potential transcriptional role of CCSP in neutrophils. |
| | |
Authors:
|
P Katavolos; C A Ackerley; M E Clark; D Bienzle |
Related Documents
:
|
9690226 - Bioreactivity and biocompatibility of a vitamin e-modified multi-layer hemodialysis fil... 1621826 - Elevation of organ resistance due to leukocyte perfusion. 12013356 - Effect of antibiotics on polymorphonuclear neutrophil apoptosis. 16488456 - Changes in peripheral blood leukocyte populations in pigs with naturally occurring exud... 7416136 - Radioxenon washout for evaluation of vasodilator therapy in mesenteric ischemia. 11834726 - Heme redox properties of s-nitrosated hemoglobin a0 and hemoglobin s: implications for ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-06 |
Journal Detail:
|
Title: Veterinary immunology and immunopathology Volume: 139 ISSN: 1873-2534 ISO Abbreviation: Vet. Immunol. Immunopathol. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1-9 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Pathobiology, University of Guelph, Ontario, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 'Theory of Mind', psychotic-like experiences and psychometric schizotypy in adolescents from the gen...
Next Document: A novel galectin-1 and interleukin 2 receptor ? haplotype is associated with autoimmune myasthenia g...