| ClC-3 chloride channels are essential for cell proliferation and cell cycle progression in nasopharyngeal carcinoma cells. | |
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MedLine Citation:
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PMID: 20539936 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ClC-3, a gene encoding a candidate protein for volume-activated chloride (C(-)) channels, may be involved in tumor development. Herein we report a study using an antisense "knock-down" strategy to investigate the mechanism by which ClC-3 affects cell proliferation in nasopharyngeal carcinoma CNE-2Z cells. With immunoblots and MTT assays we demonstrated that the expression of ClC-3 was cell cycle dependent and in a similar concentration-dependent manner, an antisense oligonucleotide specific for ClC-3 inhibited ClC-3 protein expression and cell proliferation. The expression level of ClC-3 correlated with cell proliferation. Moreover, in the cells exposed to a ClC-3 antisense oligonucleotide, the cloning efficiency was inhibited, and cells were arrested in the S phase. The ClC-3 antisense oligonucleotide inhibited the volume-activated C(-) current (I(Cl,vol)) and the regulatory volume decrease (RVD) in a concentration-dependent manner. Additionally, the I(Cl,vol) or RVD was positively correlated with cell proliferation in the treated cells. In conclusion, ClC-3 is involved in cell proliferation and cell cycle progression through a mechanism involving modulation of I(Cl,vol) and RVD. CIC-3 may represent a therapeutic target in human cancer. |
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Authors:
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Bin Xu; Jianwen Mao; Liwei Wang; Linyan Zhu; Hongzhi Li; Weizhang Wang; Xiaobao Jin; Jiayong Zhu; Lixin Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 42 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-11 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 370-80 Citation Subset: IM |
Affiliation:
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Guangdong Pharmaceutical University, Guangzhou, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma
/
metabolism*,
pathology,
physiopathology Cell Cycle Cell Line, Tumor Cell Proliferation Cell Size Chloride Channels / antagonists & inhibitors, biosynthesis, physiology* Chlorides / physiology Humans Male Nasopharyngeal Neoplasms / metabolism*, pathology, physiopathology Oligonucleotides, Antisense Patch-Clamp Techniques |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 0/Chlorides; 0/ClC-3 channel; 0/Oligonucleotides, Antisense |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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