Document Detail

ClC-3 chloride channels are essential for cell proliferation and cell cycle progression in nasopharyngeal carcinoma cells.
MedLine Citation:
PMID:  20539936     Owner:  NLM     Status:  MEDLINE    
ClC-3, a gene encoding a candidate protein for volume-activated chloride (C(-)) channels, may be involved in tumor development. Herein we report a study using an antisense "knock-down" strategy to investigate the mechanism by which ClC-3 affects cell proliferation in nasopharyngeal carcinoma CNE-2Z cells. With immunoblots and MTT assays we demonstrated that the expression of ClC-3 was cell cycle dependent and in a similar concentration-dependent manner, an antisense oligonucleotide specific for ClC-3 inhibited ClC-3 protein expression and cell proliferation. The expression level of ClC-3 correlated with cell proliferation. Moreover, in the cells exposed to a ClC-3 antisense oligonucleotide, the cloning efficiency was inhibited, and cells were arrested in the S phase. The ClC-3 antisense oligonucleotide inhibited the volume-activated C(-) current (I(Cl,vol)) and the regulatory volume decrease (RVD) in a concentration-dependent manner. Additionally, the I(Cl,vol) or RVD was positively correlated with cell proliferation in the treated cells. In conclusion, ClC-3 is involved in cell proliferation and cell cycle progression through a mechanism involving modulation of I(Cl,vol) and RVD. CIC-3 may represent a therapeutic target in human cancer.
Bin Xu; Jianwen Mao; Liwei Wang; Linyan Zhu; Hongzhi Li; Weizhang Wang; Xiaobao Jin; Jiayong Zhu; Lixin Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  42     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  370-80     Citation Subset:  IM    
Guangdong Pharmaceutical University, Guangzhou, China.
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MeSH Terms
Carcinoma / metabolism*,  pathology,  physiopathology
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Cell Size
Chloride Channels / antagonists & inhibitors,  biosynthesis,  physiology*
Chlorides / physiology
Nasopharyngeal Neoplasms / metabolism*,  pathology,  physiopathology
Oligonucleotides, Antisense
Patch-Clamp Techniques
Reg. No./Substance:
0/Chloride Channels; 0/Chlorides; 0/ClC-3 channel; 0/Oligonucleotides, Antisense

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