| Cl-Cl exchange in promyelocytic HL-60 cells follows simultaneous rather than ping-pong kinetics. | |
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MedLine Citation:
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PMID: 2782393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intra- and extracellular chloride concentration dependencies of the rate of Cl-Cl exchange in human promyelocytic leukemic HL-60 cells were studied by means of radioactive isotope (36Cl) efflux measurements. Efflux of isotope from cells follows an exponential time course. The Cl-Cl exchange flux follows Michaelis-Menten kinetics as a function of both intra- and extracellular chloride concentrations. The ratio of the maximum exchange velocity to the apparent Michaelis constant for both extracellular and intracellular substrate increases as a function of trans Cl concentration, indicating that Cl-Cl exchange in the HL-60 cell does not follow ping-pong kinetics. A kinetic scheme in which extracellular and intracellular chloride ions bind in random order to the transporter and are then translocated simultaneously can adequately model the experimental data. |
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Authors:
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D Restrepo; D J Kozody; L J Spinelli; P A Knauf |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 257 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-10-18 Completed Date: 1989-10-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C520-7 Citation Subset: IM |
Affiliation:
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Department of Biophysics, University of Rochester, School of Medicine and Dentistry, New York 14642. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anion Exchange Resins
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metabolism Biological Transport / drug effects Chlorides / metabolism*, pharmacology Humans Leukemia, Promyelocytic, Acute / metabolism, pathology* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA-07924/CA/NCI NIH HHS; DK-27495/DK/NIDDK NIH HHS; HL-18208/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anion Exchange Resins; 0/Chlorides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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