| Citicoline increases glutathione redox ratio and reduces caspase-3 activation and cell death in staurosporine-treated SH-SY5Y human neuroblastoma cells. | |
| | |
MedLine Citation:
|
PMID: 12443983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Citicoline, or CDP-choline, is an essential endogenous intermediate in the biosynthesis of phosphatidylcholine that may act as a neuroprotector in several models of neurodegeneration. The present study analyses the effects of citicoline in the paradigm of staurosporine-induced cell death in human SH-SY5Y neuroblastoma cells. Citicoline reduces apoptosis induced by 100 nM staurosporine for 12 h in SH-SY5Y cells. This effect is higher with pre-treatment of 60 mM citicoline for 24 h after staurosporine challenge. Moreover, citicoline treatment restores glutathione redox ratio diminished after staurosporine challenge. Finally, citicoline also reduces the expression levels of active caspase-3 and specific PARP-cleaved products of 89 kDa resulting from staurosporine exposure when citicoline is added to the culture medium 24 h before staurosporine. These findings demonstrate that citicoline affects the staurosporine-induced apoptosis cell-signalling pathway by interacting with the glutathione system and by inhibiting caspase-3 in SH-SY5Y human neuroblastoma cells. |
| | |
Authors:
|
Marta Barrachina; Julio Secades; Rafael Lozano; Cristina Gómez-Santos; Santiago Ambrosio; Isidro Ferrer |
Related Documents
:
|
10079203 - Liver poly(adp-ribose)polymerase is resistant to cleavage by caspases. 10229803 - Annexin v delays apoptosis while exerting an external constraint preventing the release... 10510443 - Cyclosporin a and tacrolimus (fk506) suppress expression of inducible nitric oxide synt... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Brain research Volume: 957 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2002 Dec |
Date Detail:
|
Created Date: 2002-11-21 Completed Date: 2003-03-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 84-90 Citation Subset: IM |
Affiliation:
|
Departament de Biologia Cellular i Anatomia Patològica, Universitat de Barcelona, Campus de Bellvitge, carrer Feixa Llarga sn, 08907, Hospitalet de Llobregat, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects Blotting, Western Caspase 3 Caspases / antagonists & inhibitors*, metabolism* Cell Death / drug effects Cell Line Cytidine Diphosphate Choline / metabolism, pharmacology* Enzyme Inhibitors / pharmacology* Glutathione / drug effects*, metabolism* Humans Neuroblastoma Nootropic Agents / metabolism, pharmacology* Oxidation-Reduction / drug effects Staurosporine / pharmacology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Enzyme Inhibitors; 0/Nootropic Agents; 62996-74-1/Staurosporine; 70-18-8/Glutathione; 987-78-0/Cytidine Diphosphate Choline; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pineal melatonin and brain transmitter monoamines in CBA mice during chronic oral nicotine administr...
Next Document: Environmental manipulation differentially alters c-Fos expression in amygdaloid nuclei following ave...