Document Detail


Cisplatin resistance induced by decreased apoptotic activity in non-small-cell lung cancer cell lines.
MedLine Citation:
PMID:  22397496     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We have investigated defective steps in apoptosis that might account for the development of resistance. For this purpose, A549 and Calu1 NSCLC (non-small-cell lung cancer) cell lines were treated with cisplatin to obtain resistant sub-lines. Gene expression profiles and the phosphorylation status of the BAD (Bcl-2/Bcl-XL-antagonist, causing cell death) protein were determined for each cell line. Cell death and cytochrome c release were analysed after treating cell lines with their appropriate cisplatin doses. Gene expression of BAD, Bid, caspases 4 and 6 were clearly decreased in the resistant cell lines, and the differential phosphorylation status of BAD also seemed to play a role in the development of cisplatin resistance. Since this is a new cisplatin-resistant Calu1 cell line, it is noteworthy that DNA fragmentation, apoptotic cell ratio and cytochrome c levels were most decreased in the CR-Calu1 cell line.
Authors:
Vildan B Cetintas; Ali S Kucukaslan; Buket Kosova; Aslı Tetik; Nur Selvi; Gursel Cok; Cumhur Gunduz; Zuhal Eroglu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international     Volume:  36     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  261-5     Citation Subset:  IM    
Affiliation:
*Department of Medical Biology, Ege University Faculty of Medicine, Ege University School of Medicine, 35100 Bornova, Izmir, Turkey.
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