| Cisplatin resistance induced by decreased apoptotic activity in non-small-cell lung cancer cell lines. | |
| | |
MedLine Citation:
|
PMID: 22397496 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
We have investigated defective steps in apoptosis that might account for the development of resistance. For this purpose, A549 and Calu1 NSCLC (non-small-cell lung cancer) cell lines were treated with cisplatin to obtain resistant sub-lines. Gene expression profiles and the phosphorylation status of the BAD (Bcl-2/Bcl-XL-antagonist, causing cell death) protein were determined for each cell line. Cell death and cytochrome c release were analysed after treating cell lines with their appropriate cisplatin doses. Gene expression of BAD, Bid, caspases 4 and 6 were clearly decreased in the resistant cell lines, and the differential phosphorylation status of BAD also seemed to play a role in the development of cisplatin resistance. Since this is a new cisplatin-resistant Calu1 cell line, it is noteworthy that DNA fragmentation, apoptotic cell ratio and cytochrome c levels were most decreased in the CR-Calu1 cell line. |
| | |
Authors:
|
Vildan B Cetintas; Ali S Kucukaslan; Buket Kosova; Aslı Tetik; Nur Selvi; Gursel Cok; Cumhur Gunduz; Zuhal Eroglu |
Related Documents
:
|
8422986 - Morphogenesis in drosophila requires nonmuscle myosin heavy chain function. 19450496 - Two distinct conformations of abeta aggregates on the surface of living pc12 cells. 7607076 - A dictyostelium anterior-like cell mutant reveals sequential steps in the prespore pres... 1453156 - Continuous cell movements rearrange anatomical structures in intact sponges. 16099696 - Prions and exosomes: from prpc trafficking to prpsc propagation. 16648906 - Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carci... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Cell biology international Volume: 36 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-03-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 261-5 Citation Subset: IM |
Affiliation:
|
*Department of Medical Biology, Ege University Faculty of Medicine, Ege University School of Medicine, 35100 Bornova, Izmir, Turkey. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of bovine lactoferrin on the minimum inhibitory concentrations of ampicillin and trimethoprim...
Next Document: Factors determining the effectiveness of oral ciclosporin in the treatment of severe childhood atopi...