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Cisplatin loaded PMMA: mechanical properties, surface analysis and effects on Saos-2 cell culture.
MedLine Citation:
PMID:  23748618     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: Despite wide resection and systemic chemotherapy, bone tumors may present with local recurrences, metastases and pathological fractures. Application of bone cement containing antineoplastic drug to fill the defect after resection of metastatic lesions and to support implants has been suggested to prevent local tumor growth and implant failures. In this study, we aimed to demonstrate the effects of the addition of cisplatin which is a widely used antineoplastic drug for osteosarcoma, on the mechanical properties of bone cement, and to evaluate the cytotoxic effects of eluted cisplatin on Saos-2 cell culture.
METHODS: Two cement samples were prepared by mixing 100 mg and 300 mg of cisplatin powder with 40 g cement powder. The bone cement of the control group did not contain cisplatin. Mechanical analyses included 4-point bending, compression and shear testing. For cytotoxicity analysis, samples were incubated in Dulbecco's Modified Eagle's medium for 15 days. Mediums were applied to Saos-2 cell culture and cell viability was measured. Surface analyses were performed by scanning electron microscope (SEM).
RESULTS: The addition of cisplatin did not alter the mechanical properties of bone cement. It was observed that the eluted cisplatin had cytotoxic effects on Saos-2 cells. SEM analyses demonstrated cisplatin granules on the surface of cement samples.
CONCLUSION: Cisplatin maintains its cytotoxic property when released from bone cement without compromising the mechanical stability. Application of cisplatin loaded bone cement may help local control of tumor growth. We believe that our study will shed light on to these new practices for the treatment of bone cancers and will encourage future studies.
Authors:
Hakan Ozben; Levent Eralp; Gökhan Baysal; Ayşegül Cort; Nazlı Sarkalkan; Tomris Ozben
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta orthopaedica et traumatologica turcica     Volume:  47     ISSN:  1017-995X     ISO Abbreviation:  Acta Orthop Traumatol Turc     Publication Date:  2013  
Date Detail:
Created Date:  2013-06-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9424806     Medline TA:  Acta Orthop Traumatol Turc     Country:  Turkey    
Other Details:
Languages:  eng     Pagination:  184-92     Citation Subset:  IM    
Affiliation:
Department of Hand Surgery and Microsurgery, University Hospital, University of Modena and Reggio Emilia, Modena, Italy.
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