Document Detail

Cisplatin effects on evolution of reactive oxygen species from single human bladder cancer cells investigated by scanning electrochemical microscopy.
MedLine Citation:
PMID:  22169205     Owner:  NLM     Status:  Publisher    
Reactive oxygen species (ROS) have gained great interests as they are closely related to cellular homeostasis and functions. New insights into physiology of cancer cells are anticipated from the analysis of their ROS evolution at single cell level. We discovered a periodical ROS evolution of human bladder cancer (T24) cells by time-lapse scanning electrochemical microscopy (SECM). We define one ROS generation cycle consists of one active stage when the cell is releasing ROS and one resting stage when the cell is not releasing ROS. Quantitative study of the extracellular ROS profile of a T24 cell in different stages was accomplished by comparison of experimental and simulated probe approach curves. In the active stage, the distribution of ROS around an untreated T24 cell was found to be steady with a long cycle of alternation. When apoptosis is triggered by cisplatin, the periodicity of the ROS generation cycle is significantly accelerated. Enhanced ROS productivity was observed with cisplatin-treated T24 cells in the active stage. With ROS released from the cells as the redox mediator, SECM provides an excellent label-free method to monitor the physiological activities of single cancer cells.
Michelle M N Zhang; Yi-Tao Long; Zhifeng Ding
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-26
Journal Detail:
Title:  Journal of inorganic biochemistry     Volume:  -     ISSN:  1873-3344     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905788     Medline TA:  J Inorg Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Department of Chemistry, the University of Western Ontario, London, ON, Canada N6A 5B7; Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science & Technology, Shanghai 200237, China.
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