Document Detail


Cisplatin augments FAS-mediated apoptosis through lipid rafts.
MedLine Citation:
PMID:  20651352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cisplatin is widely and effectively used for the treatment of various types of cancer. However, its biochemical mechanisms are still unelucidated. Previously, we reported that membrane sphingomyelin (SM) was important for FAS-mediated apoptosis through lipid raft function. In this study, we strikingly show that cisplatin combined with CH11 (anti-FAS antibody, IgM) was able to induce marked apoptosis in SM synthase-restored WR/Fas-SMS1 cells, but not in SM synthase-deficient WR/FAS-SM(-) cells. In addition, we demonstrated that membrane SM played an important role in cisplatin/CH11-induced apoptosis through the classical caspase-dependent pathway, mainly by enhancing the formation of FAS-associated signaling complexes.
Authors:
Cheng-Ri Huang; Zhe-Xiong Jin; Lingli Dong; Xiao-Peng Tong; Sun Yue; Takafumi Kawanami; Toshioki Sawaki; Tomoyuki Sakai; Miyuki Miki; Haruka Iwao; Akio Nakajima; Yasufumi Masaki; Yoshihiko Fukushima; Masao Tanaka; Yoshimasa Fujita; Hideo Nakajima; Toshiro Okazaki; Hisanori Umehara
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2065-71     Citation Subset:  IM    
Affiliation:
Department of Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, Ishikawa 920-0293, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD95 / physiology*
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Caspase 3 / analysis
Caspase 8 / analysis
Cell Survival / drug effects
Cisplatin / pharmacology*
Fas-Associated Death Domain Protein / physiology
Humans
Membrane Microdomains / drug effects*,  physiology
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/Antineoplastic Agents; 0/FADD protein, human; 0/Fas-Associated Death Domain Protein; 15663-27-1/Cisplatin; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8

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