Document Detail


Cis-regulatory logic driving glial cells missing: self-sustaining circuitry in later embryogenesis.
MedLine Citation:
PMID:  22509525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The glial cells missing (gcm) regulatory gene of the sea urchin Strongylocentrotus purpuratus is first expressed in veg2 daughter cells as the genomic target of late cleavage stage Delta-Notch signaling from the skeletogenic mesoderm precursors. Gcm is required in veg2 progeny during late cleavages for the early phase of pigment cell precursor specification. Here we report on a later acting cis-regulatory module that assumes control of gcm expression by the early mesenchyme blastula stage and maintains it through pigment cell differentiation and dispersal. Cis-perturbation analyses reveal that the two critical elements within this late module are consensus matches to Gcm and Six1 binding sites. Significantly, six1 mRNA localizes to gcm+cells from the mesenchyme blastula stage onwards. Trans-perturbations with anti-sense morpholinos reveal a co-dependency between six1 and gcm. Six1 mRNA levels fall sharply after Gcm is depleted, while depleting Six1 leads to significant reductions in output of endogenous gcm or modular-reporters. These results support the conclusion gcm and six1 comprise a positive intergenic feedback loop in the mesodermal GRN. This often employed cross regulatory GRN feature here ensures self-sustaining gcm output in a cohort of fully specified pigment cell precursors at a relatively early developmental stage.
Authors:
Andrew Ransick; Eric H Davidson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental biology     Volume:  364     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-16     Completed Date:  2012-05-03     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blastula / metabolism
Embryo, Nonmammalian / metabolism
Embryonic Development*
Female
Gene Expression Regulation, Developmental
Genes, Regulator
Homeodomain Proteins / genetics,  metabolism*
Mesoderm / metabolism
Molecular Sequence Data
Neuroglia / metabolism*
Strongylocentrotus purpuratus / embryology*
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
HD-37105/HD/NICHD NIH HHS; P01 HD037105/HD/NICHD NIH HHS; P01 HD037105-03/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Transcription Factors
Comments/Corrections

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