| Cis-regulatory logic driving glial cells missing: self-sustaining circuitry in later embryogenesis. | |
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MedLine Citation:
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PMID: 22509525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The glial cells missing (gcm) regulatory gene of the sea urchin Strongylocentrotus purpuratus is first expressed in veg2 daughter cells as the genomic target of late cleavage stage Delta-Notch signaling from the skeletogenic mesoderm precursors. Gcm is required in veg2 progeny during late cleavages for the early phase of pigment cell precursor specification. Here we report on a later acting cis-regulatory module that assumes control of gcm expression by the early mesenchyme blastula stage and maintains it through pigment cell differentiation and dispersal. Cis-perturbation analyses reveal that the two critical elements within this late module are consensus matches to Gcm and Six1 binding sites. Significantly, six1 mRNA localizes to gcm+cells from the mesenchyme blastula stage onwards. Trans-perturbations with anti-sense morpholinos reveal a co-dependency between six1 and gcm. Six1 mRNA levels fall sharply after Gcm is depleted, while depleting Six1 leads to significant reductions in output of endogenous gcm or modular-reporters. These results support the conclusion gcm and six1 comprise a positive intergenic feedback loop in the mesodermal GRN. This often employed cross regulatory GRN feature here ensures self-sustaining gcm output in a cohort of fully specified pigment cell precursors at a relatively early developmental stage. |
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Authors:
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Andrew Ransick; Eric H Davidson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Developmental biology Volume: 364 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-04-16 Completed Date: 2012-05-03 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 259-67 Citation Subset: IM |
Affiliation:
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California Institute of Technology, Pasadena, CA 91125, USA. andyr@caltech.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blastula / metabolism Embryo, Nonmammalian / metabolism Embryonic Development* Female Gene Expression Regulation, Developmental Genes, Regulator Homeodomain Proteins / genetics, metabolism* Mesoderm / metabolism Molecular Sequence Data Neuroglia / metabolism* Strongylocentrotus purpuratus / embryology* Transcription Factors / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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HD-37105/HD/NICHD NIH HHS; P01 HD037105-03/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/Transcription Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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