Document Detail

Cis-2-decenoic acid inhibits S. aureus growth and biofilm in vitro: a pilot study.
MedLine Citation:
PMID:  22585351     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cis-2 decenoic acid (C2DA) disperses biofilm in many strains of microorganisms. However, whether C2DA inhibits bacterial growth or has potential to boost the actions of antibiotics is unknown.
QUESTIONS/PURPOSES: We asked whether (1) C2DA inhibited MRSA growth and biofilm, (2) antibiotics increased inhibitory effects, (3) inhibitory concentrations of C2DA were cytotoxic to human cells, and (4) effective concentrations could be delivered from a chitosan sponge drug delivery device.
METHODS: Broth containing seven concentrations of C2DA and six concentrations of either daptomycin, vancomycin, or linezolid was inoculated with a clinical isolate of MRSA and added to a total of 504 coated microtiter plate wells in triplicate (n = 3) for turbidity bacterial growth and crystal violet biofilm mass quantification. We used fibroblast cell viability assays of six C2DA concentrations (n = 4) to evaluate preliminary biocompatibility. We measured the elution of C2DA from a chitosan sponge drug delivery device with two representative loading concentrations (n = 3).
RESULTS: C2DA at concentrations of 500 μg/mL and above inhibited growth, while 125 μg/mL C2DA inhibited biofilm. Combination with antibiotics increased these effects. At concentrations up to 500 μg/mL, there were no cytotoxic effects on fibroblasts. Chitosan sponges loaded with 100 mg of C2DA eluted concentrations at or above biofilm-inhibitory concentrations for 5 days.
CONCLUSIONS: C2DA inhibited biofilm formation by MRSA at biocompatible concentrations, with increasing biofilm reduction with added antibiotics. Elution of C2DA from a chitosan sponge can be modified through adjusting loading concentration.
CLINICAL RELEVANCE: By inhibiting biofilm formation on implant surfaces, C2DA may reduce the number of infections in musculoskeletal trauma.
Jessica Amber Jennings; Harry S Courtney; Warren O Haggard
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical orthopaedics and related research     Volume:  470     ISSN:  1528-1132     ISO Abbreviation:  Clin. Orthop. Relat. Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-14     Completed Date:  2012-11-19     Revised Date:  2013-10-11    
Medline Journal Info:
Nlm Unique ID:  0075674     Medline TA:  Clin Orthop Relat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2663-70     Citation Subset:  AIM; IM    
Department of Biomedical Engineering, University of Memphis, Memphis, TN, USA,
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MeSH Terms
Biofilms / drug effects*,  growth & development*
Fatty Acids, Monounsaturated / pharmacology*
Methicillin-Resistant Staphylococcus aureus / drug effects*,  growth & development*
Microbial Sensitivity Tests
Pilot Projects
Reg. No./Substance:
0/Fatty Acids, Monounsaturated; 332T8TH7B1/2-decenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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