Document Detail

Circulating neutrophils in Behçet disease is resistant for apoptotic cell death in the remission phase of uveitis.
MedLine Citation:
PMID:  17690898     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Behçet disease (BD) is manifested by recurrent acute iridocyclitis with hypopyon in the active phase, which regresses spontaneously. Hypopyon consists of inflammatory cells infiltrating the eye, with polymorphonuclear cells (PMNs) as the main component. The present study was conducted to investigate the apoptosis property of PMNs in BD patients with uveitis. METHODS: PMNs were purified from peripheral blood cells of BD patients with uveitis in the active or remission phase and were cultured for 12 hours. In some cultures, lipopolysaccharide (LPS), antagonistic anti-TNFalpha antibody, agonistic anti-Fas antibody, or Fas:Fc fusion protein was added. At the end of cultures, apoptotic cells were evaluated by Annexin V expression using flow cytometry. RESULTS: Spontaneous apoptosis of PMNs showed lower levels in the remission phase of BD-related uveitis compared with the active phase or healthy controls. The lower level of PMN apoptosis in the remission phase of uveitis in BD remained even by stimulation with LPS, anti-TNFalpha antibody, or Fas:Fc fusion protein, which was abolished in the presence of agonistic anti-Fas antibody. CONCLUSIONS: In BD patients, the apoptosis of PMNs was reduced in the remission phase of uveitis and restored in the active phase, which arose from the apoptotic cell death in part via Fas-Fas ligand interaction.
Keita Fujimori; Keiko Oh-i; Masaru Takeuchi; Naoyuki Yamakawa; Takaaki Hattori; Takeshi Kezuka; Hiroshi Keino; Jun Suzuki; Hiroshi Goto; Jun-ichi Sakai; Masahiko Usui
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-10
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie     Volume:  246     ISSN:  0721-832X     ISO Abbreviation:  Graefes Arch. Clin. Exp. Ophthalmol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-18     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-90     Citation Subset:  IM    
Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan.
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MeSH Terms
Annexin A5 / metabolism
Antigens, CD95 / metabolism
Apoptosis* / drug effects
Behcet Syndrome / blood*,  immunology,  pathology
Cell Culture Techniques
Fas Ligand Protein / metabolism
Flow Cytometry
Lipopolysaccharides / pharmacology
Middle Aged
Neutrophils / immunology,  pathology*
Tumor Necrosis Factor-alpha / immunology
Uveitis, Anterior / blood*,  immunology,  pathology
Reg. No./Substance:
0/Annexin A5; 0/Antigens, CD95; 0/FAS protein, human; 0/Fas Ligand Protein; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha

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