| Circulating levels of apelin, glucagon-like peptide and visfatin in hypercholesterolemic-hyperhomocysteinemic guinea-pigs: their relation with NO metabolism. | |
MedLine Citation:
|
PMID: 25380625 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of this study was to determine the levels of regulatory peptides apelin, glucagon-like peptide (GLP-1) and visfatin in hypercholesterolemic and hyperhomocysteinemic state and to examine their relation with nitric oxide (NO) metabolism. 32 Male guinea pigs were divided into four groups and each group was fed as follows: (a) commercial chow, (b) cholesterol (chol)-rich diet, (c) methionine (meth)-rich diet, and (d) chol + meth-rich diet. Blood samples were drawn at the end of 10 weeks, and abdominal aorta was dissected for histopathological examination. Serum insulin, GLP-1, apelin, visfatin, and nitrotyrosine concentrations were measured by the manufacturer's kits based on ELISA; asymmetric dimethylarginine (ADMA) and arginine levels were measured by the high performance liquid chromatography. Homocysteine level was measured by the chemiluminescence immunoassay; glucose, total chol and triglyceride levels were measured by the autoanalyzer. The microscopic examination of aorta indicated varying degrees of vascular disturbance in chol- and chol + meth-fed groups. High levels of chol and homocysteine, accompanied with significantly low levels of apelin and GLP-1 were detected in the plasma. Visfatin, ADMA, and nitrotyrosine levels both in chol- and chol + meth-fed groups were significantly higher than those in control animals, whereas arginine and arginine/ADMA ratio were lower. This study indicated that circulating levels of apelin, GLP-1, and visfatin are markedly altered during the development of atherosclerotic changes in close association with chol, homocysteine, NO, and ADMA levels. The measurements of these peptides in serum may help for the diagnosis and follow-up of vascular dysfunction. |
Authors:
|
Zeynep Kusku-Kiraz; Sema Genc; Seldag Bekpinar; Yesim Unlucerci; Vakur Olgac; Mujdat Uysal; Figen Gurdol |
Related Documents
:
|
6867015 - Influence of malnutrition and alterations in dietary protein on murine rotaviral disease. 12170615 - Effect of dietary protein and calorie deficiency on tryptophan levels in the developing... 7299495 - Effect of maternal diet on ganglioside distribution in fetal rat brain. 238215 - Effect of maternal protein deprivation on enzymatic development in newborn rats. 23134625 - Increase in standard cholesterol and large hdl particle subclasses in antiretroviral-na... 15489205 - Dietary habits and growth and recurrence of colorectal adenomas: results from a three-y... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2014-11-8 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: - ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2014 Nov |
Date Detail:
|
Created Date: 2014-11-8 Completed Date: - Revised Date: 2014-11-9 |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of negative emotions evoked by light, noise and taste on trigeminal thermal sensitivity.
Next Document: Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by s...