Document Detail


Circulating immune complexes in patients with rheumatoid arthritis and the effect of penicillamine in vitro.
MedLine Citation:
PMID:  6723125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein precipitated from the sera of 15 patients with rheumatoid arthritis (RA) by increasing concentrations of polyethylene glycol 6000 (PEG) was compared with that from 20 normal controls. The wide range of precipitable protein obtained was only significantly different for the two groups when a 4% PEG concentration was used. To increase the discrimination of the procedure, the precipitated protein was measured for IgG, IgM, C3 and C4 using immunospecific antisera and laser nephelometry. The data obtained from each of these procedures did not increase the specificity of the technique as a means of distinguishing between patients and controls. As part of an investigation of the effect of penicillamine on immune complexes, penicillamine was incubated at 37 degrees C for 24 and 48 h with sera containing immune complexes from RA patients. When the immune complexes were precipitated with PEG, it was found that incubation, by itself, reduced the amount of PEG precipitable protein. The results of in vitro incubation with penicillamine were very variable.
Authors:
J C Crawhall
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and investigative medicine. Médecine clinique et experimentale     Volume:  7     ISSN:  0147-958X     ISO Abbreviation:  Clin Invest Med     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-09     Completed Date:  1984-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7804071     Medline TA:  Clin Invest Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigen-Antibody Complex / analysis*
Arthritis, Rheumatoid / drug therapy*,  immunology
Complement C3 / analysis
Complement C4 / analysis
Humans
Immunoglobulin G / analysis
Immunoglobulin M / analysis
Penicillamine / therapeutic use*
Rheumatoid Factor / analysis
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Complement C3; 0/Complement C4; 0/Immunoglobulin G; 0/Immunoglobulin M; 52-67-5/Penicillamine; 9009-79-4/Rheumatoid Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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