Document Detail


Circulating endothelial progenitor cells in congestive heart failure.
MedLine Citation:
PMID:  17070610     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Endothelial progenitor cells (EPCs) circulate in the adult peripheral blood and contribute to neovascularization. EPCs are considered to be included in CD34 positive mononuclear cells (CD34+ MNCs). Kinetics of circulating EPCs in congestive heart failure (CHF) has not been fully investigated. METHODS: We determined the numbers of white blood cells (WBCs), plasma brain natriuretic peptide (BNP), serum erythropoietin, vascular endothelial growth factor (VEGF) and thrombomodulin levels in 16 mild CHF patients (NYHA I, II), 10 severe CHF patients with acute exacerbation (NYHA III, IV), and 22 control subjects. The number of CD34+ MNCs in peripheral blood was quantified by flow cytometry. RESULTS: The ratio of CD34+ MNCs:10(3) WBCs in mild CHF patients was higher than that in control subjects (P<0.05). Interestingly, the ratio of CD34+ MNCs:10(3) WBCs in severe CHF patients at admission was significantly lower than that in control subjects (P<0.005) or in mild CHF patients (P<0.05). Levels of BNP and erythropoietin in severe CHF patients were significantly higher than those in mild CHF patients. However, VEGF and thrombomodulin levels were not different between mild and severe CHF patients. In addition, the ratio of CD34+ MNCs:10(3) WBCs in severe CHF patients increased in proportion to the amelioration of CHF during hospitalization, and this increase correlated with the decrease in BNP level. CONCLUSIONS: The ratio of CD34+ MNCs:10(3) WBCs was decreased in severe CHF. These findings suggest that impaired EPC recruitment might be involved in the pathophysiology of severe CHF.
Authors:
Mutsuko Nonaka-Sarukawa; Keiji Yamamoto; Hirotaka Aoki; Yoshioki Nishimura; Hidenori Tomizawa; Masaru Ichida; Takayuki Eizawa; Kazuo Muroi; Uichi Ikeda; Kazuyuki Shimada
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-27
Journal Detail:
Title:  International journal of cardiology     Volume:  119     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-25     Completed Date:  2007-07-31     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  344-8     Citation Subset:  IM    
Affiliation:
Division of Cardiovascular Medicine, Jichi Medical University, Shimotsuke, Tochigi 329-0498, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aged
Antigens, CD34*
Case-Control Studies
Erythropoietin / blood
Female
Heart Failure / blood*
Humans
Leukocyte Count
Leukocytes, Mononuclear*
Male
Middle Aged
Natriuretic Peptide, Brain / blood
Severity of Illness Index
Thrombomodulin / blood
Vascular Endothelial Growth Factor A / blood
Chemical
Reg. No./Substance:
0/Antigens, CD34; 0/Thrombomodulin; 0/Vascular Endothelial Growth Factor A; 11096-26-7/Erythropoietin; 114471-18-0/Natriuretic Peptide, Brain

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