Document Detail

Circulating endothelial cells in coronary artery disease.
MedLine Citation:
PMID:  20967702     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: endothelial damage and dysfunction play a crucial role in the pathophysiology of coronary artery disease (CAD). The quantification of circulating endothelial cells (CEC) in the peripheral blood is a novel method for assessing endothelial damage.
AIM: to evaluate the possible diagnostic use of single quantification of CEC in peripheral blood by flow cytometry in patients with CAD.
METHODS: we examined 48 patients with CAD, including 23 patients with acute myocardial infarction (AMI) and 25 patients with stable angina (SA). The control group consisted of 20 healthy subjects without symptoms of CAD. The CEC count was evaluated by flow cytometry using antibodies against CD31, CD146, and CD45. Plasma biochemical markers of endothelial damage (von Willebrand Factor [vWF], thrombomodulin [TM]) were measured by ELISA. Serum concentrations of troponin I (TnI) and lipid parameters were also included in the statistical analysis.
RESULTS: A significant increase in the CEC count was found in patients with AMI compared to the control group (p < 0.05) and SA patients (p < 0.05). However, no difference was found in the CEC count between patients with SA and the control group. Increased vWF activity was found in both groups of CAD patients compared to the control group (AMI: p < 0.001, SA: p , 0.01), and vWF activity was significantly higher in AMI patients compared to SA patients (p < 0.001). Thrombomodulin concentration did not differ significantly between any patient groups and the control group. The CEC count correlated positively with vWF activity (r = 0.3852, p < 0.05) and the atherogenic index TC/HDL-C (r = 0.3844, p < 0.05) in all patients with CAD (AMI + SA). The sensitivity of CEC count for the diagnosis of an acute coronary syndrome was lower than that of TnI level on admission (39% vs 69%).
CONCLUSIONS: we confirmed that CEC count in peripheral blood can be determined by flow cytometry in CAD patients with both AMI and SA. The CEC count in AMI was increased in comparison to healthy subjects and SA patients in one third of all cases. To determine whether CEC count could be used to improve the diagnosis of an acute coronary syndrome in patients with CAD, additional studies in larger patient groups would be required.
Magdalena Lampka; Zofia Grąbczewska; Ewa Jendryczka-Maćkiewicz; Iga Hołyńska-Iwan; Adam Sukiennik; Jacek Kubica; Waldemar Halota; Tomasz Tyrakowski
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Kardiologia polska     Volume:  68     ISSN:  0022-9032     ISO Abbreviation:  Kardiol Pol     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-22     Completed Date:  2011-05-31     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  0376352     Medline TA:  Kardiol Pol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  1100-5     Citation Subset:  IM    
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MeSH Terms
Angina Pectoris / blood*,  pathology*
Antigens, CD146 / blood
Antigens, CD31 / blood
Antigens, CD45 / blood
Biological Markers / blood
Coronary Circulation
Endothelial Cells / pathology*
Endothelium, Vascular / pathology*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Middle Aged
Myocardial Infarction / blood*,  pathology*
Troponin I / blood
Reg. No./Substance:
0/Antigens, CD146; 0/Antigens, CD31; 0/Biological Markers; 0/Troponin I; EC, CD45
Comment In:
Kardiol Pol. 2010 Oct;68(10):1106   [PMID:  20967703 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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