| Circulating cycloxygenase-2 in patients with tobacco-related intraoral squamous cell carcinoma and evaluation of its peptide inhibitors as potential antitumor agent. | |
| | |
MedLine Citation:
|
PMID: 20213098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: The aim of this study was to quantitate circulating COX-2 levels in patients with tobacco-related intraoral cancer and to evaluate antitumor activities of COX-2 peptide inhibitors in vitro on KB cell lines. PATIENTS AND METHODS: We used a novel biosensor-based surface plasmon resonance (SPR) technique for estimation of circulating COX-2 levels in 76 patients with oral cancer and 43 normal individuals. Antitumor activities of five COX-2 inhibitory peptides were evaluated using propidium iodide labeling and flow cytometry, alamar blue, MTS, and annexin-V binding assays. RESULTS: Patients with oral cancer showed threefold increase in serum COX-2 level when compared to normal controls (P < 0.0001). Further, late-stage tumors and lymph node metastasis were associated with significant increase in serum COX-2 levels. Patients with higher circulating COX-2 also showed higher immunoreactivity to anti-COX-2 antibody in the lesions. The peptides significantly reduced viability and inhibited growth/proliferation, induced cytotoxicity and apoptosis in tumor cells. However, no such effect was observed either on normal human leukocytes or on MCF-7 cell line that did not over express COX-2. CONCLUSION: Our results indicate that SPR may be a useful proteomic technique for quantitative assessment of COX-2 and to identify patients with high-risk oral premalignant or occult cancer, as well as in monitoring response to novel COX-2 targeting strategies. Furthermore, COX-2 peptide inhibitors appear to be a new class of potent anticancer agent for human oral carcinoma. |
| | |
Authors:
|
Vaishali Kapoor; Abhay K Singh; Sharmistha Dey; Suresh C Sharma; Satya N Das |
Related Documents
:
|
15623598 - Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identi... 17007818 - Pharmacological inhibition of poly(adp-ribose) polymerase inhibits angiogenesis. 12431818 - Angiogenesis in prostate cancer: its role in disease progression and possible therapeut... 11900248 - Colorectal cancer prevention and treatment by inhibition of cyclooxygenase-2. 18723368 - Optimizing treatment for men with advanced prostate cancer: expert recommendations and ... 11267858 - Prognostic importance of the soluble plasminogen activator receptor, supar, in plasma f... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-06 |
Journal Detail:
|
Title: Journal of cancer research and clinical oncology Volume: 136 ISSN: 1432-1335 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-10-25 Completed Date: 2010-11-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: Germany |
Other Details:
|
Languages: eng Pagination: 1795-804 Citation Subset: IM |
Affiliation:
|
Tumor Biology and Molecular Immunology Laboratory, Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Antineoplastic Agents / chemical synthesis, pharmacology Apoptosis / drug effects Carcinoma, Squamous Cell / enzymology*, etiology, prevention & control Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Cyclooxygenase 2 / blood, metabolism* Cyclooxygenase Inhibitors / chemical synthesis, pharmacology Female Flow Cytometry Humans Immunohistochemistry Lymphatic Metastasis Male Middle Aged Mouth Neoplasms / enzymology*, etiology, prevention & control Neoplasm Staging Peptides / chemical synthesis, pharmacology* Smoking / adverse effects Surface Plasmon Resonance Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Cyclooxygenase Inhibitors; 0/Peptides; EC 1.14.99.1/Cyclooxygenase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA methylation and carcinogenesis of PRDM5 in cervical cancer.
Next Document: Clinical investigation of malignant mesothelioma in Japan.