Document Detail

Circulating cell-free fetal messenger RNA levels after fetoscopic interventions of complicated pregnancies.
MedLine Citation:
PMID:  16626602     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: The aim of this study was to examine fetal gene expression in maternal plasma after fetoscopic intervention for twin-twin transfusion syndrome or congenital diaphragmatic hernia. STUDY DESIGN: Twelve women with pregnancies that were complicated by twin-twin transfusion syndrome and 10 women carrying fetuses with congenital diaphragmatic hernia were sampled before and sequentially after treatment. Levels of glyceraldehyde-3-phosphate dehydrogenase, human placental lactogen, and gamma globin messenger RNA were measured by real-time reverse transcriptase polymerase chain reaction amplification. RESULTS: At all time points, glyceraldehyde-3-phosphate dehydrogenase messenger RNA levels were higher in the congenital diaphragmatic hernia cases than in the twin-twin transfusion syndrome cases (P < .05), but during the immediate postoperative observation period, there were no significant changes in glyceraldehyde-3-phosphate dehydrogenase, human placental lactogen, or gamma globin messenger RNA levels in individual patients or patients who were grouped by procedure. CONCLUSION: Fetoscopic intervention of complicated pregnancies does not affect circulating fetal messenger RNA levels, which is in contrast to earlier observations that circulating fetal DNA levels increase after laser ablation for twin-twin transfusion syndrome. Plasma glyceraldehyde-3-phosphate dehydrogenase messenger RNA levels could be a potential novel biomarker for fetal trauma.
May Lee Tjoa; Jacques Jani; Liesbeth Lewi; Inga Peter; Tuangsit Wataganara; Kirby L Johnson; Diana W Bianchi; Jan A Deprest
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-04-19
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  195     ISSN:  1097-6868     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  230-5     Citation Subset:  AIM; IM    
Department of Pediatrics, Division of Genetics, Tufts-New England Medical Center, Boston, MA 02111, USA.
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MeSH Terms
Fetal Diseases / surgery*
Fetofetal Transfusion / surgery*
Gene Expression
Globins / analysis
Glyceraldehyde-3-Phosphate Dehydrogenases / blood
Hernia, Diaphragmatic / congenital,  surgery*
Laser Coagulation / methods*
Placental Lactogen / analysis
Polymerase Chain Reaction
RNA, Messenger / analysis*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 9004-22-2/Globins; 9035-54-5/Placental Lactogen; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases

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