| Circulating cell-free fetal DNA for the detection of RHD status and sex using reflex fetal identifiers. | |
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MedLine Citation:
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PMID: 23225162 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the sensitivity and specificity of circulating cell-free fetal DNA in determining the fetal RHD status and fetal sex. METHODS: Maternal blood was collected in each trimester of pregnancy from RhD negative nonalloimmunized women. Whole blood was centrifuged, separated into plasma and buffy coat, and frozen at -80°C. DNA analysis was conducted via allele-specific primer extensions for exons 4, 5, and 7 of the RHD gene and for a 37-base pair insertion in exon 4 (RHD pseudogene; psi) three Y-chromosome sequences (SRY, DBY, and TTY2), and an extraction control (TGIFL-like X/Y). RhD serotyping on cord blood and gender assessment of the newborns were entered into a Web-based database. RESULTS: One hundred twenty women were enrolled. The median gestational age at the first venipuncture was 12.4 (range: 10.6-13.9) weeks with 120 samples drawn; 118 samples were drawn at 17.6 (16-20.9) weeks; and 113 samples at 28.7 (27.9-33.9) weeks. Overall accuracy for RHD was 99.1%, 99.1%, and 98.1% for each trimester and was 99.1%, 99.1%, and 100% for fetal sex determination. CONCLUSIONS: Fetal RHD genotyping and sex can be very accurately determined in all three trimesters using circulating cell-free fetal DNA in the maternal circulation. © 2012 John Wiley & Sons, Ltd. |
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Authors:
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K J Moise; N H Boring; R O'Shaughnessy; L L Simpson; H M Wolfe; J K Baxter; W Polzin; K A Eddleman; S S Hassan; D Skupski; G McLennan; T Paladino; P Oeth; A Bombard |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-6 |
Journal Detail:
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Title: Prenatal diagnosis Volume: - ISSN: 1097-0223 ISO Abbreviation: Prenat. Diagn. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: - |
Other Details:
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Languages: ENG Pagination: 1-6 Citation Subset: - |
Copyright Information:
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© 2012 John Wiley & Sons, Ltd. |
Affiliation:
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Baylor College of Medicine, Houston, TX, USA; University of Texas School of Medicine at Houston, Houston, TX, USA. Kenneth.J.Moise@uth.tmc.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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